摘要
目的:深入理解超声微泡介导靶向治疗的机制,了解其研究现状,对实现基因和药物的定向治疗,并将其应用于临床有重要意义。资料来源:应用计算机检索Pubmed数据库1995-01/2006-01有关超声微泡介导靶向治疗的文章,检索词“Microbubble,gene,drug”,限定文章语言种类为English。同时计算机检索中国期刊全文数据库、万方数据1994-01/2006-01期间的相关文章,检索词“超声微泡、基因、治疗”,限定文章语言种类为中文。资料选择:资料初审后,选取符合研究要求的文章查找全文。纳入标准:①有关超声微泡介导靶向治疗的研究。②有关超声微泡系统在肾脏病领域的研究。排除标准:重复研究。资料提炼:共收集到67篇有关超声微泡介导靶向治疗的文章,排除重复或类似研究,32篇符合研究要求。资料综合:①超声微泡介导靶向治疗的主要机制:声波的空化效应导致细胞膜通透性增高,使微泡所携带的基因或药物进入组织细胞内,从而提高基因的转染效率及局部药物浓度。②超声微泡介导靶向治疗的研究现状:国内外超声微泡携带基因治疗的方法已应用于心血管、神经系统等领域的试验研究,表明该基因治疗方法是一种安全、高效的体内基因定位转染新技术。另外,采用微泡运送溶栓和抗肿瘤药物,可提高靶组织的药物浓度,降低全身毒副作用。③超声微泡介导系统在肾脏病中的应用:超声微泡系统已成功将目的基因转染多种肾脏病动物模型,转染效率明显提高,并有效保护肾功能,减轻肾脏纤维化。④研究展望:深入研究,制备高效、安全的靶向微泡造影剂,优化超声参数,超声微泡系统将具有广阔的应用前景。结论:超声微泡介导靶向治疗可增强基因转染效率,提高特定组织的基因表达水平和药物浓度,是一种安全、简便、高效的靶向性基因转染及药物治疗的新方法。
OBJECTIVE： To further explore the mechanism and research status of uhrasound-microbubble-mediated system for target therapy, so as to provide insights into application of gene and medicine target therapy in clinical experience. DATA SOURCES： A computer-based online search of Pubmed database was undertaken for articles on microbubble and target therapy published in English between January 1995 and January 2006 with the key words of ＂Microbubble, gene, drug＂. Meanwhile, we searched CNKI and WangFang database for related articles published in Chinese between January 1994 and January 2006 with the same key words in Chinese. STUDY SELECTION： The data were checked up firstly, and the full- texts of the papers met the requirements were found. Inclusive criteria： ① Study about microbubble-mediated target therapy. ② Study about uhrasound-microbubble system in renal disease. Exclusive criteria： repetitive study. DATA EXTRACTION： Sixty-seven articles about ultrasound microbubble-mediated target therapy were collected. The repetitive or similar study was excluded and 32 articles accorded with inclusive criteria were included. DATA SYNTHESIS： ①Mechanism of ultrasound-microbubble-mediated system： The increase of cellular membrance permeability caused by cavatition erosion of uhrasonic wave brings gene or medicine into special tissue, which enhances gene transfection efficiency and medicine concentration of target tissue. ② Research status of ultrasound- microbubble-mediated system： The microbubble-mediated gene therapy has been applied into cardiovascular and nervous system in experiments, indicating that microbubble-mediated system is a safe and efficient gene location transfection method. In addition, microbubble with thrombolysis and anti-tumor medicine may increase medicine concentration in target tissue and lessen side effects. ③Application of uhrasound-microbubble system in renal disease： Gene transfection with high efficiency that was achieved by uhrasound-microbubble-mediated system in many animal models of renal diseases may inhibit inflammation, prevent renal function impairment and attenuate renal fibrosis. ④ Prospects of this therapy： Uhrasound-microbubble-mediated system will be used widely if the problems below are resolved： To prepare high-performance and safe targeted microbubble; optimize ultrasound parameter and so on. CONCLUSION： Uhrasound-microbubble-mediated target therapy, which can promote gene transfer efficiency, enhance transgene expression and medicine concentration in special tissue, is a safe convenient and effective method for target therapy and will be widely applied in clinical experience.
出处
《中国临床康复》
CSCD
北大核心
2006年第45期148-151,共4页
Chinese Journal of Clinical Rehabilitation
作者简介
叶琨，女，1975年生，江苏省丹徒县人，汉族，中南大学湘雅二医院肾内科在读博士，主要从事肾小球疾病及肾间质纤维化方面的研究。
通讯作者：刘伏友，教授，博士生导师，中南大学湘雅二医院肾内科，湖南省长沙市410011
