摘要
目的 建立开胸顺气滴丸中厚朴酚与和厚朴酚含最的测定方法。方法 采用RP—HPLC法，色谱柱为VPC18（150mm×4．6mm，5μm）；流动相为甲醇-乙腈-水（50：20：40）；流速1.0ml·min^-1；柱温25℃；检测波长294nm。结果 厚朴酚的线件范围为0．2961～2．9610μg（r=0.9996）；和厚朴酚的线性范围为0．1608—1．6080μg（r=0．9996）。厚朴酚的平均回收率为99.17％±1．66％，RSD=1．3％（n=6）；和厚朴酚的平均回收率为99．04％±2.06％，RSD=1．7％（n=6）。结论 所用方法简便、准确，可用于开胸顺气滴丸中厚朴酚与和厚朴酚的含量测定。
OBJECTIVE To establish a method for determination of magnolol and isomagnololin in Kaixiong Shunqi drops. METH- ODS An RP - HPLC method was adopted. The analysis was carried out on an analytical column C18 （ 150 mm × 4. 6 mm,5 μm）. The mobile phase consisted of methanol -acetonitrile -water （50 ： 20 ： 40 ） and the flow rate was 1.0 ml·min^-1, The detective wavelength was set at 294 nm with column temperature of 25℃. RESULTS The lienar range was 0. 2961 - 2. 9610 μg （ r = 0. 9996） and the average recovery was 99.17%±1.66% with the RSD of 1.3% （n = 6） for magnolol, respectively. The linear range was 0, 1608 - 1. 6080 μg（ r = 0. 9996 ） and the average recovery was 99.04%± 2.06% with the RSD of 1.7% （ n = 6 ） for isomagnololin, respectively. CONCLUSION The method is simple,reproducible and suitable for determination of magnolol and isomagnololin in Kaixinng Shunqi drops.
出处
《华西药学杂志》
CAS
CSCD
北大核心
2006年第6期577-579,共3页
West China Journal of Pharmaceutical Sciences
作者简介
蒋平（1971-），男．四川阆中，硕士，主管药师。从事中药质量标准的制定工作。
