摘要
从GenBank检索并下载了我国近40年的麻疹病毒血凝素蛋白(H)和核蛋白(N)的全基因组序列,用Bioedit7.0软件对参考株和代表株进行序列比对,并进行氨基酸进化树的构建,进一步从氨基酸层面分析麻疹流行株的变化速率。结果显示麻疹病毒H蛋白发生的变异,表现为近十年的变异率(1.33×10-3)大约为前三十年变异率(0.95×10-3)的1.4倍,而N蛋白发生的变异,表现为近些年的变异率(1.90×10-3)大约为前三十年变异率(1.01×10-3)的1.8倍。本研究结果提示麻疹病毒近年变异率有增快的趋势,今后需加强对麻疹病毒基因型变异和演变的监测。
The gene sequences of the Hemagglutinin and Nucleoprotein were downloaded from GenBank. Bioedit7.0 softwere was used to pairwise these sequences and to construct a phylogenetic tree to analyze the variation rate of measles virus strains. The results demonstrated that variation rate of hemagglutinin gene was 1.33×10^-3 in the recent 10 years, which was about 1.4-fold higher than in the former 30 years. The variation rate of nucleoprotein gene was 1.90×10^-3 in the recent 10 years, which was about 1.8-fold higher than in the former 30 years. These findings suggest that monitoring the variation of measles virus should be emphasized because of the increasing variation rate in the recent 10 years.
出处
《中国病毒学》
CSCD
2006年第6期541-545,共5页
Virologica Sinica
基金
浙江省自然科学基金项目(Y204458)
关键词
麻疹
基因
变异速率
Measles
Gene
Variation rate
作者简介
李海峰（1976-），男，浙江省籍，主治医师，从事临床研究。
通讯作者：卢亦愚（1948-），男，浙江省籍，教授，从事病毒学研究。Corresponding author,Tel：0571—87235034，E-mail：luyjyuzjh@yahoo．com．cn
