摘要
目的研究腹腔镜手术中二氧化碳(CO2)气腹环境对恶性卵巢肿瘤细胞黏附、侵袭能力的影响,探讨恶性肿瘤行腹腔镜手术的安全性。方法体外培养人卵巢癌SKOV3细胞,模拟腹腔镜CO2气腹环境处理肿瘤细胞,噻唑蓝比色(MTT)法和Boyden小室侵袭运动实验检测SKOV3细胞的黏附、运动和侵袭能力;免疫组化和半定量RT-PCR方法检测SKOV3细胞黏附分子CD44及促血管生成因子bFGF的蛋白和mRNA表达。结果与对照组比较,CO2处理组细胞对人工基底膜的黏附力明显增加(P<0.01);处理组细胞运动和侵袭能力明显增强(P<0.01);其CD44和bFGF蛋白分泌和mRNA表达量显著增加(P<0.05)。结论CO2气腹环境能促进体外培养的恶性卵巢肿瘤细胞的侵袭转移,其作用机制可能与其促进卵巢癌细胞异质黏附和运动能力;增加细胞黏附分子和促血管生成因子的表达有关。
Objective To investigate the effects and mechanism of carbon dioxide （CO,） pneumoperitoneum on the adhesive, migratory and invasive abilities of ovarian carcinoma cell line SKOV3. Methods SKOV3cells were exposed to an CO2 insufflation medium. The cells＇ adhesive ability to Matrigel was analyzed by MTF assayand, and their migratory and invasive abilities were analyzed through Boyden filters metastasis model. CD44 and bFGF expressions were examined by immunohistochemistry and semi-quantitative RT-PCR. Results Cells exposed to an in vitro pneumoperitoneum demonstrate could significantly increase adhesive, migratory and invasive abilities compared to the control group （P 〈 0.01 ）. The CD44 and bFGF expressions were significantly increased too （P 〈0.05）. Conclusion Carbon dioxide pneumoperitoneum may improve the adhesive, migratory and invasive abilities of ovarian carcinoma cell line in vitro, probably by increasing the expressions of adhesion and invasion relative molecules .
出处
《第三军医大学学报》
CAS
CSCD
北大核心
2006年第23期2380-2382,共3页
Journal of Third Military Medical University
关键词
CO2
气腹
卵巢肿瘤
黏附分子
肿瘤侵袭
carbon dioxide pneumoperitoneum
ovarian tumor
adhesiveness
tumor invasion
作者简介
唐均英（1964-），女，重庆市人，博士，副教授、主要从事妇科腹腔镜方面的研究，发表论文15篇.电话：（023）89012325。
