摘要
从免疫鸡群中分离到9株新城疫病毒（NDV），其中5株为强毒株，4株为中强毒株。用RT—PCR扩增F基因cDNA片段，并将其分别克隆到pGEM—Teasy载体中。序列分析结果表明，上述分离株F基因长度为1662bp，编码553个氨基酸，其F蛋白的氨基酸序列同源性为96．0％～99．8％；但与常见疫苗株的氨基酸同源性仅为87.6％～92.2％，F蛋白裂解位点序列为^112R-R-Q／R—K—R—FI—G^119,均属于基因Ⅶ型NDV。
Nine strains of Newcasttle disease virus（NDV） were isolated from immunized chicken flocks, 5 isolates of which were velogenic and the others were mesogenic. The cDNA fragments of F gene of 9 NDV strains above were amplified by RTPCR. The amplified fragments were cloned into pGEM-Teasy vector and the recombinant plasmids were sequenced. The results showed that the F gene from all of the NDV isolates consisted of 1 662 bp ,coding for 553 amino acids. The amino acid sequence homology among the 9 NDV isolates came to 96.0%-99.8% ,but the amino acid sequence homology between the 9 isolates and other NDV vaccine strains（LaSota,B1,F48E9 and Roakin） were relatively lower,from 87.6% to 92.2%. The sequence of cleavege site of F protein were ^112R-R-Q/R-K-R-F-I-G^119,all of the 9 isolates belonged to the genetype Ⅶ.
出处
《中国兽医学报》
CAS
CSCD
北大核心
2006年第6期597-599,共3页
Chinese Journal of Veterinary Science
作者简介
刘铀（1966-）,男,副教授,博士。
