摘要
目的探讨陕西汉族人群间隙连接蛋白37(Connexin 37,Cx37)基因多态性与冠心病的关系。方法采用聚合酶链反应和限制性片段内切酶的方法检测了150例冠心病患者(冠状动脉造影证实)及117例对照者的Cx 37基因多态性。结果冠心病患者T等位基因频率高于对照组(0.25vs0.21),两组间3种基因型(TT、TC和CC)分布无显著性差异。在男性群体中,冠心病患者T等位基因频率高于对照组(0.254vs0.243),3种基因型(TT、TC和CC)分布在两组男性间,无显著性差异。结论汉族人群中存在Cx37基因多态性,其多态性与冠心病发病无关。
AIM To investigate the association between Connexin 37 （Cx37） gene polymorphism and coronary heart disease （CHD） in population of Shaanxi Han nationality. METHODS Samples of peripheral blood white cells were extracted from 150 patients with CHD, aged 61 ± 10, and from 117 controis without cardiovascular diseases, aged 60 ± 8. The subjects under study were all of Chinese Han nationality. The Cx 37 polymorphism was determined by polymerase chain reaction-restriction fragment length polymorphism （PCR-RFLP）. RESULTS The frequency of the Cx37 gene T allele （0.25 vs 0.21 ） was higher in the CHD group than that in the controls, but the frequencies of three genotypes were not significantly different in both groups . In the male group, the frequency of the Cx37 gene T allele （0.254 vs 0. 243 ） was higher in the CHD group than that in the controls, but the frequencies of three genotypes were not significantly different in both groups . CONCLUSION The polymorphism of Cx37 gene is present in population of Shaanxi Han nationality and there is no association between the polymor- phism of Cx37 gene and CHD.
出处
《心脏杂志》
CAS
2006年第5期539-541,共3页
Chinese Heart Journal
基金
西京医院科技创新基金项目资助(No.XJCX03M011)
作者简介
谢学建，住院医生，硕士Tel：（029）82554075 Email：xxuejian@eyou．com
通讯作者：郭文怡，主任医师，主要从事冠心病基础与临床研究Tel：（029）84775183 Email：guowenyi@163．net
