摘要
目的探讨细胞因子在CD4^+CD25^+调节性T细胞免疫抑制机制中的作用,观察胃癌患者外周血中的CD4^+CD25^+调节性T细胞及其效应性T细胞CD4^+CD25^-T细胞产生具有不同生物活性的细胞因子的水平。方法采用免疫磁珠分选方法分离胃癌患者外周血中的CD4^+ CD25^+T细胞与CD4^+CD25^-T细胞后,将CD4^+CD25^+T细胞与效应性T细胞CD4^+CD25^-T细胞在体外分别单独培养及按不同比例共同培养后,再用酶联免疫吸附试验(ELISA)法检测单独及混合培养时细胞因子干扰素(IFN)-γ、白细胞介素(IL)-10及转化生长因子(TGF)-β分泌的水平。结果健康对照及胃癌患者CD4^+CD25^+T细胞分泌抑制性细胞因子IL-10、TGF-β均显著高于CD4^+CD25^-T细胞,而分泌细胞因子IFN-γ均显著低于CD^+CD25^-T细胞(P＜0．01)。CD4^+ CD25^+T细胞在体外可明显抑制CD4^+CD25^-T细胞产生IFN-γ的能力,且这种抑制能力呈效靶比关系(P＜0．01)；但CD4^+CD25^+T细胞与效应性T细胞CD4^+CD25^-T细胞在体外共培养对IL- 10及TGF-β的分泌无显著影响(P＞0．01)。结论CD4^+CD25^+调节性T在体外可能通过细胞因子的调节发挥对效应性T细胞的抑制作用。
Objective To explore the role of cytokines in immune suppression mechanism of CD4 ^＋ CD25^ ＋ regulatory T cells and detect the level of cytokines secreted by the CD4 ^＋ CD25 ^＋ regulatory T cells and CD4^＋ CD25^- T cells in peripheral blood of gastric cancer patients. Methods After CD4^＋ CD25^ ＋ T cells and CD4 ^＋ CD25^- T cells were isolated by magnetic activated cell sorting （MAC,S）, CD4^ ＋ CD25 ^＋ T cells and CD4^＋ CD25^- T cells were cultured separately or co-cultured in different ratios. The IFN-γ, IL-10 and TGF-α levels of CD4^ ＋ CD25 ^＋ T and CD4^ ＋ CD25^- T cells in vitro were determined by ELISA. Results The levels of suppressive cytokines （IL-10 and TGF-fl） secreted by the CD4 ＋ CD25 ＋ T cells in the gastric cancer patients and healthy controls were significantly higher than CD4 ＋ CD25- T cells,but the level of proliferative cytokine IFN-~＇ was much lower than CD4＋ CD25- T cells （P 〈 0.01）. CD4 ＋ CD25 ＋ T cells could obviously inhibit the IFN-~＇ production by CIM ＋ CD25- T cells in vit- ro （P〈 0.01） in an effect-ratio relationship. However, co-culture of CD4＋ CD25＋ T cells and CD4＋ CD25- T cells had no effect on IL-10 and TGF-13 production （P 〉 0.01）. Conclusion The cytokines may have some effects in the immune suppression mechanism of CD4＋ CD25 ＋ regulatory T cells in vitro.
出处
《中华实验外科杂志》
CAS
CSCD
北大核心
2006年第11期1375-1377,共3页
Chinese Journal of Experimental Surgery
关键词
胃癌
T细胞
细胞因子
Gastric carcinoma
T cells
Cytokine
