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溶组织内阿米巴ELISA试剂盒在阿米巴病调查的应用 被引量:6

Epidemiological survey of amoebiasis by using TECHLAB EHISTOLYTICAL Ⅱ ELISA kits
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摘要 目的了解景洪市爱尼族人群阿米巴病流行情况,为阿米巴病监测和防治提供依据,并评价ELISA试剂盒在阿米巴病调查应用的效果。方法以整群随机抽样爱尼族村寨,逐户收集新鲜粪便标本,用观察法、碘液涂片镜检法和TECHLAB第二代溶组织内阿米巴ELISA试剂盒对照检测粪便标本溶内阿米巴。结果镜检278例,E·h/E·d感染为9·71%;ELISA检测366例,E·h感染率为9·29%;镜检和ELISA共同阳性15例,占镜检阳性的55·56%;粪便观察和ELISA检测结果诊断5例为肠阿米巴病,发病率为1·37%,E·h致病率为14·71%。结论景洪爱尼族阿米巴病流行严重,应加强阿米巴病的监测和防治工作,同时积极推广新技术。 Objective To understand the prevalent situation of amoebiasis among Aini ethnic group in Jinghong City, Yunnan Province and provide basis for monitoring and control of the disease. Methods An epidemiologieal survey was carried out by random cluster sampling Aini ethnic group villages and fecal samples were collected house by house. The fecal samples were detected under microscope with iodine staining, TECHLAB 2^nd generation Monoclonal ELISA kits. Results The infection rates of E. histolytica and E dispar were 9.71% among 278 persons examined by microscope with iodine staining. The infection rate of E. histolytica was 9.29% among 366 eases detected by ELISA. There were 15 ELISA positives among a total of 27 positives examined by microscope with iodine staining, E. histolytica accounted for 55.56% of E. histolytica / E dispar. 5 intestinal amoebiasis eases were diagnosed by stool examination and ELISA with an incidence of 1.37 % and the pathogenic rate of E. h istolytica was 14.71%. Conclusion Amoebiasis prevails seriously among Aini ethic group in Jinghong City. The control and monitoring measures should be strengthened and new diagnostic technique be applied simultaneously.
出处 《中国热带医学》 CAS 2006年第11期1931-1932,共2页 China Tropical Medicine
关键词 阿米巴病 爱尼族 溶组织内阿米巴/迪斯帕内阿米巴 溶组织内阿米巴 TECHLAB第二代溶组织内阿米巴EUSA试剂盒 Amoebiasis Aini ethnic group E. histolytica/E dispar E, histolytica TECHLAB E. HISTOLYTICAL Ⅱ ELISA kits
作者简介 孙晓东(1973-),男,云南人,主管医师,本科,主要从事寄生虫防治工作.
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  • 1刘伟,中国寄生虫病防治杂志,1993年,6卷,300页
  • 2程训佳,中国寄生虫学与寄生虫病杂志,1993年,11卷,172页
  • 3Haque R, Kress K, Wood S, et al. Rapid diagnosis of Entamoeba infection by using Entamoeba and Entamoeba histolytica stool antigen detection kits[J]. J Clin Microbiol,1995,33: 2558-2561.
  • 4Karki BMS, Parija SC. Co-agglutination test for the detection of circulation antigen in amebic liver abscess[J]. Am J Med Hyg,1999,60(3) :498-501.
  • 5Bhaskar S, Singh S, Sharma M, A single-step immunochromatographic test for the detection of Entamoeba histolytica antigen in stool samples[J]. J Immunological Methods, 1996,196:193-198.
  • 6Jackson TFHG. Entamoeba histolytica and Entamoeba dispar are distinct species: Clinical, epidemiological and serological evidence[J]. International Journal for Parasitology,1998,28:181-186.
  • 7Ramos F, Valenznela A, Moran P, et al. Anti-E. histolytica IgA antibodies in saliva of E. histolytica and E. dispar infected individuals:longitudinal study of cohorts[J]. Arch Med Res, 1997,28: s372-s329.
  • 8Gomes MA, Pesquero JB, Furst C, et al. An improved method to distinguish Entamoeba histolytica and Entamoeba dispar[J]. Parasitology, 1999,199:395-362.
  • 9Clark CG, Dlamond LS. Intraspecific variation and phylogenetic relationships in the genus Entamoeba as revealed by riboprinting[J]. J Eukaryot Microbiol, 1997,44:42-154.
  • 10Britten D, Wilson SM, Menerney R, et al. An improved colormetric PCR-based method for detection and differentiation of Entamoeba histolytica and Entamoeba dispar in feces[J]. J Clin Microbiol, 1997,35:1108-1111.

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  • 1郭增柱,祝宏,安亦军,王正仪.多聚酶链反应在阿米巴病流行病学研究中的应用[J].中华流行病学杂志,1995,16(5):303-305. 被引量:4
  • 2李丽,张再兴,周升.溶组织阿米巴的流行、诊断和治疗(英文)[J].中国热带医学,2006,6(5):904-905. 被引量:2
  • 3陈家旭,常正山.ELISA检测粪抗原诊断寄生虫感染的研究进展[J].国际医学寄生虫病杂志,2006,33(6):296-300. 被引量:17
  • 4WHO. Amoehiasis [J]. Weekly Epidemiological Record, 1997, 72(14): 97-100.
  • 5WHO/PAHO/UNESCO. A consultation with experts on amoebi asis: Mexico City, Mexico 28-29 January, 1997[J].Epidemio Bull, 1997, 18(1):13-4.
  • 6Haque R, Mollah Nu, Ali Ikm, et al. Diagnosis of amebic liver abscess and intestional infection with the Techlab Enamoeba histolytica antigen detection and antibody tests [J]. J Clin Microbiol, 2000, 38(9):3235-9.
  • 7WHO. Amoebiasis [J]. Weekly Epidemiological Record, 1997, 72: 97--100.
  • 8WHO/PAHO/UNESCO report: a consultation with experts on amoebiasis: Mexico City, Mexico 28-29 January, 1997[J]. Epi- demiol Bull, 1997, 18(1):13--14.
  • 9Haque R, Mollah N, Ali I, et al. Diagnosis of amebic liver ab- scess and intestional infection with the Techlab Enamoeba histo-lytica antigen detection and antibody tests [J]. J Clin Microbiol, 2000, 38(9):3235--9.
  • 10蒋则孝,许隆祺,余森海,徐淑惠,周长海,许景田,常江,王云昆.中国溶组织内阿米巴感染的状况[J].中国寄生虫病防治杂志,1997,10(4):264-268. 被引量:16

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