摘要
目的 寻找一种简单可靠的方法制作大鼠慢性酒精性肝病模型。方法 给大鼠饮用5％递增到22％浓度的酒精，然后再以54％酒精每日3次，每次1．2～1．5mL灌胃的方法连续5或10周建立大鼠慢性酒精性肝病模型；常规HE染色，光镜观察大鼠肝脏病理学形态改变，并检测血清ALT、AST水平。结果 酒精灌胃5周后，40％（8／20）大鼠发生肝脂肪变性，酒精灌胃10周后，85％（17／20）大鼠发生肝脂肪变性，45％（9／20）大鼠出现酒精性肝炎的病理变化；酒精灌胃5、10周后，大鼠血清ALT、AST分别为（61±16）、（81±20）和（90±16）、（130±32）U·L^-1，均较同期对照组（33±8）、（58±9）和（46±15）、（51±11）U·L^-1有显著升高（P〈0．05）；酒精灌胃10周后的大鼠血清ALT、AST较酒精灌胃5周后升高（P〈0．05）。结论 采用梯度浓度酒精、分次少量灌胃的方法，成功地制作了大鼠慢性酒精性肝病模型。
Objective To search a simple and reliable method for establishing an alcoholic liver disease model in rats. Methods Rats drank alcohol ad libitum with increasing concentrations from 5% to 22%. Then 1.2 - 1.5 mL of 54% alcohol was given to the animal by intragastric injection t. i. d. for 5 or 10 weeks. The morphological changes of the liver were observed by using light microscopy and HE staining. Results At the end of the fifth week（5 wk group）, 40% （8/20）of the rats had mild and moderate steatosis. At the end of the tenth week（ 10 wk group）, 85% （17/20） had liver steatosis and 45% （9/20） had alcoholic hepatitis. The serum level of ALT and AST in ethanol - treated mrs was significantly higher than that of controls [5 wk： （61±16）, （81 ±20）U·L^-1 vs（33 ±8）, （58 ± 9）U·L^-1 respectively, P 〈 0.05; 10 wk：（90±16）, （130 ±32）U·L^-1 vs （46±15 ）, （51±11 ）U·L^-1 respectively, P 〈 0.05 ]. 10 wk group was higher than 5wk group（ P 〈 0.05）. Conclusion Chronic alcoholic liver disease in rat was established by intragastric injection with small quantity of gradually increasing concentrations of alcohol into the rats for several times a day.
出处
《解放军预防医学杂志》
CAS
北大核心
2006年第5期336-338,共3页
Journal of Preventive Medicine of Chinese People's Liberation Army
关键词
酒精性肝病
酒精灌胃法
肝功能
肝病模型
alcoholic liver disease
intragastric injection with alcohol
hepatic function
liver disease model
作者简介
伏建峰（1967-），男，医学硕士，副主任技师。从事临床生化研究。
