摘要
利用随机扩增DNA（RAPD）技术分析了15个大豆疫霉菌（Phytophthora sojae）的遗传多样性，并同其它7个疫霉种的8个菌株进行了比较。运用筛选出的12个引物共获得152个RAPD标记，其中84．2％具有多态性。通过聚类分析结果表明．疫霉属种间的遗传相似系数的变化幅度为0．533—0．783，大豆疫霉菌与其它几种疫霉的遗传距离相对较远，用引物OPB06扩增到P．sojae种特有的一个RAPD标记；P．sojae种内菌株间遗传相似系数的变化幅度为0．855—0．967，亦存在较丰富的遗传多样性。该遗传多样性与菌株毒力之间有一定相关性，而与菌株地理来源没有相关性。
Random amplified polymorphic DNA markers were used to assess genetic diversity among 15 isolates of Phytophthora sojae and 8 isolates of seven other Phytophthora species. 152 RAPD markers were obtained, which were amplified by 12 random declaimer primers, with 84.2% polymorphisms. Using cluster analysis, the genetic diversity among Phytophthora species was detected, and RAPD similarity coefficients ranged from 0. 533 to 0. 783. P. sojae was far away from seven other Phytophthora species in genetic distances. Moreover, a special RAPD marker of P. sojae was amplified by primer OPB06. RAPD analysis also indicated that there existed genetic diversity within P. sojae isolates, with similarity coefficient between 0. 855 and 0. 967. There was no correlation between DNA polymorphism of P. sojae isolates and their geographical origin, but the relationship between virulence pattern and RAPD markers was presented in some isolates.
出处
《中国油料作物学报》
CAS
CSCD
北大核心
2006年第3期330-334,共5页
Chinese Journal of Oil Crop Sciences
基金
黑龙江省重点攻关项目(G97B2-3-01)
作者简介
陈宏宇（1976-），女，现在北京有机农业发展中心工作。
通讯作者：jzhwen@sohu．com。
