摘要
利用通用引物,对大豆蚜Aphis glycinesMatsumura的细胞色素氧化酶Ⅱ(COⅡ)基因序列进行克隆和测序。测得的序列长度为810 bp,并与已在GenBank上登录的其他蚜虫COⅡ基因序列进行比较分析,验证其同源性,同时将该序列在GenBank进行了登记(登录号为DQ265743)。根据此片段的碱基序列设计了1对大豆蚜特异引物,其扩增片段约为270 bp;种特异性检验结果表明,该引物只对大豆蚜具有扩增能力,对其他相关蚜虫种类不具有扩增效果;并用此引物对大豆蚜捕食性天敌进行定性检测,结果表明,在取食过大豆蚜的异色瓢虫Harmonia axyridis、龟纹瓢虫Propylaeajaponica、大草蛉Chrysopa septempunctata幼虫以及小花蝽Orius similes成虫和幼虫等捕食性昆虫的中肠中均能检测到大豆蚜的DNA片段;而在未取食蚜虫的上述天敌中却未能扩增出来。
Cytochrome oxidase subunit Ⅱ gene segment of Aphis glycines was cloned and sequenced. The length of the sequence was 810 bp other aphids registered at GenBank primer was developed, and its this pair of primer showed the （GenBank accession no. DQ265743） to identify their molecular homology. amplified single band was about 270 bp. ~ The sequence was contrasted with One pair of sequence-characterized The specificity tests performed with presence of the band only in A. glycines. Molecular identification of the natural enemies fed on A. glycines was tested with the specific primer. The results indicated that the target DNA fragment of A. glycines was detected in guts of the predatory insects Harmonia axyridis, Propylaea japonica, Chrysopa septempunctata, and Orius similes, but not detected in the above predatory insects without feeding A. glycines.
出处
《昆虫学报》
CAS
CSCD
北大核心
2006年第5期754-758,共5页
Acta Entomologica Sinica
基金
黑龙江省"十一五"攻关重点项目(GB06B105)
关键词
大豆蚜
细胞色素氧化酶Ⅱ基因
克隆
天敌
捕食性昆虫
分子鉴定
Aphis glycines
cytochrome oxidase subunit Ⅱ gene
clone
natural enemy
predatory insects
molecular identification
作者简介
高红秀，女，1980年9月生，硕士研究生，E-mail：gaohongxiu_1999@163．com
通讯作者 ，E-mail：kjzhao@neau．edu．cn
