摘要
本文合成了二个模拟阿维菌素（ＡｖｅｒｍｅｃｔｉｎＢ_１）特征结构的半抗原，４″－Ｏ－（单）琥珀酰阿维菌素和５－Ｏ－（单）琥珀酰阿维菌素。将半抗原与载体蛋白（ＢＳＡ、ＯＶＡ和ＫＬＨ）连接，制成人工抗原。用４″－Ｏ－（单）琥珀酰阿维菌素－ＢＳＡ免疫家兔，制备针对阿维菌素的多克隆抗体。以间接ＥＬＩＳＡ方法对抗体进行了鉴定。
The polyclonal anti-Avermectin B_1(AVM)antibodies were developed. 4″-0-succinoyl AVM(hapten)and 5-O-succinoly AVM(hapten)were synthetized successfully. The haptens were coupled to BSA, OVA and KLH by NHS ester procedure. The antibodies were obtained successfully by immunizing New Zealand rabbits with 4″-0-succinoyl AVM-BSA.The indirect competitive ELISA for AVM was established. The antibodies had high specificity and avidity for AVM(I50=5.6ng/ml),and can also recognized Ivermectin(I50=10.0ng/ml).
出处
《畜牧兽医学报》
CAS
CSCD
北大核心
1996年第6期531-538,共8页
ACTA VETERINARIA ET ZOOTECHNICA SINICA
关键词
阿维菌素
抗寄生虫药
抗体
酶联免疫吸附
Avermectin B_1, Antiparasitic agent, Antibodies, Enzyme-linked immunosorbent assay (ELISA)
