摘要
依据干扰素(IFN)分子、磷脂分子本身的理化性质和结构特点,分别用三种制备方法,以四种脂质体为膜材,制备IFN脂质体,考察了不同膜材、不同制备方法对脂质体粒径及包封率的影响.结果表明,以二肉豆蔻酰胆碱和二棕榈酰磷脂酰胆碱复合材料为主要膜材,采用薄膜蒸发法制备的IFN脂质体有良好的稳定性,60d内其粒径可以保持在200～350nm,包封率可保持30%～40%.
According to the physical and chemical characteristics of interferon and Lecithin, we selected four kinds of lecithin compounds as the liposomal membrane materials, prepared liposomes by three methods, investigated liposomal shape, size distribution and the entrapment efficiency （EE） for a 60 d period to explore their influence factors of stability. The result shows the liposome made from DMMPC ＋ DPPC ＋ SA by film evaporation method has a good EE and size stability for 2 months. The size range is from 200 nm to 300 nm, the EE range is from 30%—40%.
出处
《高等学校化学学报》
SCIE
EI
CAS
CSCD
北大核心
2006年第10期1986-1990,共5页
Chemical Journal of Chinese Universities
关键词
脂质体
干扰素
薄膜蒸发法
粒径分析
Liposome
Interferon
Film evaporation method
Size distribution
作者简介
曹淑桂（1944年出生），女，教授，博士生导师，主要从事化学与生物药物研究．E-mail：caosg@jlu．edu．cn
