摘要
无细胞毒性浓度的粉防己碱（０．５ｍｇ·Ｌ－１），明显地增强三尖杉酯碱对人早幼粒白血病ＨＬ６０耐药细胞的生长抑制作用，降低集落形成率，但对敏感ＨＬ６０细胞没有增强作用。ＤＰＨ荧光标记法测定表明，粉防己碱对细胞膜流动性没有影响；提取经药物作用后的细胞ＤＮＡ，琼脂糖凝胶电泳显示，粉防己碱增加三尖杉酯碱引起的ＨＬ６０耐药细胞ＤＮＡ降解，使细胞以编程方式死亡。结果表明：粉防己碱能逆转人早幼粒白血病ＨＬ６０耐药细胞对三尖杉酯碱的耐药性，值得临床上进一步研究。
The non-cytotoxic concentration of tetrandrine(Tet, i.5mg·L-1)effatively poteintiatal the growth-inhibitory action of harringtonine(Har) in human promyelocytic leukemia HL60 resistant cells and decreased the plating efficiency of the cells. But Tet had no poteitiating effect on the sensitive HL60 cells. Tet did not disturb the cell membrane fluidity by determination with 1, 6-diphenyl-1, 3, 5 hexatriene (DPH) fluorescent labeling. Having been extracted genomic DNA from the cells treated by the drugs,the"ladder" of DNA appeared in agarose gel electropheresis when the cells were treated with Har+Tet, which was the hallmark of programmed cell death. The results demonstrated that tetrandrine can reverse the harringtonine resistance in the human promyelocytic leukemia HL60 cells and will be worthful for further clinical research.
出处
《北京师范大学学报（自然科学版）》
CSCD
1996年第2期262-265,共4页
Journal of Beijing Normal University(Natural Science)
基金
国家自然科学基金
国家教委开放实验室基金
关键词
粉防己碱
三尖杉酯碱
早幼粒白血病
HL60细胞
tetrandrine
harringtonine
human promyelocytic leukemia HL60 cells
drug resistance
programmed cell death
