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PCR及相关技术在鸡球虫种株鉴别方面的应用 被引量:6

Application of Techniques Based on PCR in the Differentiation of Eimeria Species and Strain from Chickens
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摘要 鸡球虫病是危害养禽业的重大疫病之一,其病原属于艾美耳属(Eimeria)的球虫,世界上公认有7种。过去,其病原的鉴别一直是研究的难点。近年来,由于分子生物学技术,尤其是PCR及其相关技术的迅猛发展及在寄生虫分类中的应用,鸡球虫种、株鉴别方法也由以形态特征和生活史等为标准的传统分类学进入了以核酸为材料的现代分子分类学时代。文章对PCR及其相关技术在鸡球虫种、株鉴别方面的应用做了较为全面的阐述,并分析了其优缺点。 Chicken coccidiosis, one of the important diseases, severely endangers poultry industry. Its pathogens belong to Eimeria. In the past , the pathogenic differentiations were very difficult. With the rapid development of molecular biological techology and the application in parasite taxology,the strain differentiation of Eimeria species and strain from chickens entries the molecular biological taxology from the traditional taxology. The review describes systemically the application and progress of molecular biological methods based on PCR in the differentiation of Eimeria species and strain from chickens, and analyzes briefly their advantages and disadvantages.
出处 《动物医学进展》 CSCD 2006年第9期18-22,共5页 Progress In Veterinary Medicine
基金 安徽科技学院引进人才专向基金项目(ZRC200678) 安徽科技学院校科研项目(2006YZ001)
关键词 球虫 聚合酶链式反应 Eimeria polymerase chain reaction(PCR) chicken
作者简介 李文超(1979-),男,河南南阳人,讲师,硕士,主要从事兽医寄生虫病的病原生物学与防治技术研究。
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参考文献14

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二级参考文献1

  • 1M. W. Shirley,N. Bumstead. Intra-specific variation withinEimeria tenella detected by the random amplification of polymorphic DNA[J] 1994,Parasitology Research(4):346~351

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