摘要
目的对云南省1例诊断为“间日疟”患者血片中形态不典型的疟原虫虫种进行分子生物学鉴定。方法分别抽提待鉴定血片和已知感染虫种的4种疟原虫(间日疟原虫、恶性疟原虫、诺氏疟原虫、食蟹猴疟原虫)血片疟原虫基因组DNA,再根据疟原虫小核糖体亚基(SSUrRNA)序列合成疟原虫属特异性引物,以及恶性疟原虫、间日疟原虫、诺氏疟原虫种特异性引物,然后对包括待鉴定血片在内的疟原虫DNA分别进行PCR鉴定。结果用诺氏疟原虫特异性引物从待鉴定血片DNA中扩增出约150bp条带,测序结果表明该序列与诺氏疟原虫SSUrRNA序列完全一致。结论云南省该例疟疾患者感染了猴疟原虫——诺氏疟原虫。
Objective To confirm the diagnosis of a human case with atypical vivax-malaria from Yunnan Province by molecular technique. Methods DNA was extracted from blood films of unidentified sample, and of four known Plasmodium species （P. vivax, P. falciparum, P. knowlesi, and P. cynomolgi）, A DNA-based diagnosis with the polymerase chain reaction （PCR） method targeting the small subunit ribosomal RNA （SSU rRNA） genes of genus- and species-specific （two human malaria species and P. knowlesi） was introduced. Results The PCR amplification with primer pair specific for P. knowlesi produced a single fragment of 150 bp. Sequence analysis showed that the amplified fragment was identical to the sequence of P. knowlesi. Conclusion The patient was naturally infected with P. knowlesi.
出处
《中国寄生虫学与寄生虫病杂志》
CAS
CSCD
北大核心
2006年第4期273-276,共4页
Chinese Journal of Parasitology and Parasitic Diseases
基金
国家科技部"自然资源平台建设项目(No.2004DKA30480
2005DKA21104)~~
作者简介
通讯作者．E—mail：zhuhm22@yahoo．com
