摘要
目的:探讨SLE患者T细胞亚群的FasFasL信号传导通路与T细胞凋亡紊乱的关系。方法:应用流式细胞术测定T细胞亚群表面Fas、FasL的表达率及细胞质中活化caspase3的表达率。结果:与健康对照组相比较,活动期及稳定期SLE患者组CD4+T细胞表面Fas的表达率均显著增加(P<0.05),CD8+T细胞表面Fas的表达率略有增加但无统计学意义(P>0.05)。稳定期和活动期SLE患者组T细胞亚群表面FasL的表达率均显著增加(P<0.05),但两疾病组间T细胞亚群表面Fas、FasL的表达率无显著性差异(P>0.05)。活动期SLE患者组T细胞亚群细胞质中活化caspase3的表达率,明显高于稳定期SLE患者组和健康对照组(P<0.05)。稳定期SLE患者组T细胞亚群细胞质中活化caspase3的表达率略高于健康对照组,但无统计学意义。结论:SLE患者外周血T细胞亚群凋亡加速,CD4+T细胞的凋亡活跃,其中FasFasL信号传导途径可能起重要的作用。T细胞凋亡紊乱的程度与SLE的活动程度密切相关。
AIM： To explore the relationship between Fas-FasL-mediated signal transduction pathway and apoptosis disorder of T lymphocyte subset in SLE patients. METHODS： The expression rate of membrane Fas, FasL and that of intracellular activated caspase-3 of T lymphocyte subset were determined by flow cytometry. RESULTS： Compared with healthy control group, the expression rate of membrane Fas on CD4^＋ T cells significantly increased in SLE patients in the active and inactive phases （P〈0.05）, however, that on CD8^＋ T cells slightly increased but there was no statistical significance （ P 〉 0.05 ）. The expression rate of FasL on T cell subset in SLE patients in the active and inactive phases significantly increased （ P 〈 0.05 ） but there was no obvious difference of the expression rate of Fas and FasL on T cell subset between the two disease groups （P 〉 0.05 ）. The expression rate of intracellular activated caspase-3 in T cell subset of SLE patients in the active phase was notably higher than that in the inactive phase and healthy control group （P〈0.05）. The expression rate of intracellular activated caspase-3 in T cell subset of SLE patients in the inactive phase was slightly higher than that in health control group but there was no statistical significance. CONCLU- SION： Apoptotic speed of T lymphocyte subset in SLE patients was accelerated while CD4^＋ T cells were in a state of active apoptosiso It is possible that Fas-FasL signal trans-duction pathway plays an important role in the induction of T cell apoptosis. The degree of apoptosis of T lymphocytes closely correlates with the disease＇s activity in SLE patients.
出处
《细胞与分子免疫学杂志》
CAS
CSCD
北大核心
2006年第5期588-590,593,共4页
Chinese Journal of Cellular and Molecular Immunology
基金
国家自然科学基金资助项目(30470683)
四川省科技厅项目基金(04SG022011)
作者简介
陈雪（1980-），女，四川雅安人．硕士
Correspondingauthor．Tel：028．85422751 E-mail：Wangll87@sohu．com
