摘要
目的探讨血管内皮生长因子(VEGF)对脊髓损伤细胞凋亡的影响及保护机制。方法采用Allen的Weightdropping法挫伤大鼠T10节段脊髓,于术后2、4、8h,1、3、7d经蛛网膜下隙导管各注入VEGF溶液(5μl/100g),并与生理盐水组和正常组作对照。采用原位末端标记法标记脱氧核糖核酸片段,检测脊髓损伤前后发生凋亡的脊髓细胞。结果正常对照组大鼠脊髓中未见凋亡细胞。生理盐水组于伤后4h始出现凋亡细胞。VEGF组与生理盐水组相比较,凋亡细胞明显减少(P<0.01)。结论VEGF可抑制脊髓损伤后细胞的凋亡,从而保护损伤的脊髓组织。
Objective： To explore the effect of vascular endothelial growth factor （VEGF） on cell apoptosis after spinal cord injury （SCI） and the proteetive meehanism of VEGF in injured spinal eord. Methods：Fifty-throe Wistar rats were randomly divided into 3 groups ：eontrol group （ receiving lamineetomy; n = 5 ）, saline group （ subaraehnoid injection of saline ; n = 24 ） , and VEGF group （ subaraehnoid injection of VEFG of 5 μl/100 g; n =24）. The SCI rat model was established by using Allen＇ s weight dropping method, and the thoracic vertebra （T10） was injured. The apoptotie eells were detected by TUNEL staining 2, 4, and 8 hours and 1, 3, and 7 days after SCI. Results：No apoptotie eell was found in eontrol group. The apoptotie eells were found 4 hours after SCI in saline group. Compared with saline group, the number of apoptotie eells was signifieandy less in VEGF group at eaeh time point （ P 〈 0.01 ）. Conelusion：VEGF may proteet injured spinal eord by suppressing the eell apoptosis after SCI.
出处
《中国医科大学学报》
CAS
CSCD
北大核心
2006年第3期267-268,共2页
Journal of China Medical University
关键词
血管内皮生长因子
脊髓损伤
细胞凋亡
vascular endothelial growth factor
spinal cord injury
apoptosis
作者简介
段有文（1967-）,男,副主任医师,博士.现在沈阳医学院附属奉天医院.研究方向：脊髓损伤病因学.
通讯作者 吕刚,E-mail：ganglv_sy@163.com.
