摘要
以我国云南和四川产的5个块菌菌株为试验材料,提取块菌DNA并对rDNA的ITS序列分析试验条件的初步研究,结果表明:运用常规CTAB法提取块菌子实体DNA,获得的DNA产量高,可以满足本试验的要求;明确了rDNA ITS区域的适宜PCR扩增和克隆条件;获得4个长度为650bp、1个长度为800bp的PCR产物;将PCR产物连接到PMD18-T载体上,转化E.coliJM109,获得了阳性克隆,供进一步序列分析用。
5 truffle samples clustered from Sichuan and Yunnan were selected to extract DNA and the internal transcribed spacer （ITS） regions of nuclear ribosomal DNA was amplified. The results showed： The optimal PCR and clone conditions were assured; The length of 4 samples was 650bp andl sample was 800bp; The PCR product was linked to PMD18-T vector and transformed into E.coliJM109. We chose the positive clone which would use in the further sequence analysis.
出处
《中国食用菌》
2006年第5期37-39,共3页
Edible Fungi of China
基金
农业部"98"资助项目(项目编号2001-21)
作者简介
王晓娥（1979-），女，助教
通讯作者：E-mail：yaofi@yahoo．com．cn
