摘要
目的探讨子痫前期患者胎盘组织中转化生长因子β1(TGF-β1)、血管细胞黏附分子1(VCAM-1)和E选择素(E-selectin)的表达变化及其意义。方法用免疫组化链霉菌抗生物素蛋白-过氧化物酶连接(SP)法,对20例正常孕妇(对照组)和40例子痫前期患者(子痫前期组,其中轻度16例、重度24例)的胎盘组织进行TGF-β1、VCAM-1和E-selectin定位,并用计算机图像分析系统进行定量分析比较。结果(1)1FGF-β1在胎盘绒毛合体滋养细胞的表达,子痫前期组为70．7±0．5,对照组为70．3±0．6,两组比较,差异有统计学意义(P<0．05)。VCAM-1的表达,子痫前期组为82．5±0．5,对照组为82．8±0．3;E-selectin的表达,子痫前期组为53．5±0．5,对照组为53．8±0．4;两组分别比较,差异均有统计学意义(P<0．05)。TGF-β1、VCAM-1、E-selectin在轻度子痫前期患者胎盘组织合体滋养细胞中的表达分别为70．6±0．6、82．4±0．6、53．4±0．5,在重度子痫前期患者胎盘组织中的表达分别为70．8±0．4、82．6±0．5、53．6±0．5,轻度与重度子痫前期患者TGF-β1、VCAM-1、E- selectin表达水平比较,差异均无统计学意义(P>0．05)。(2)E-selectin在胎盘绒毛毛细血管内皮细胞的表达,子痫前期组为63．0±0．5,对照组为62．6±0．4,两组比较,差异有统计学意义(P<0．05);轻度子痫前期患者为63．2±0．4、重度子痫前期为62．9±0．5,二者比较,差异无统计学意义(P> 0．05)。结论TGF-β1、VCAM-1和E-selectin在胎盘组织中的表达变化,在子痫前期发病中有重要作用。
Objective To study the change and significance of the expression of transforming growth factor-beta 1 （ TGF-β1 ） , vascular cell adhesion molecule-1 （ VCAM-1 ） and endothelium-selectin （ E- selectin） in placenta of patients with pre-eclampsia. Methods Twenty normal pregnant women （ control group） and 40 women with pre-eclampsia（pre-eclampsia group, including 16 women with mild pre-eclampsia and 24 women with severe pre-eclampsia） were selected. The cellular distribution of TGF-β1, VCAM-1 and E-selectin in placenta in both groups was determined by immunohistochemistry, and the mean density was measured by computer image analysis system. Results （ 1 ） The level of TGF-β1 in placental villous syncytiotrophoblast of pre-eclampsia group （ 70. 7 ± 0. 5 ） was significantly higher than that of control group （70. 3 ± 0. 6） , while the level of VCAM-1 and E-selectin in pre-eclampsia group （ VCAM-1 ： 82. 5 ± 0. 5, E-selectin： 53. 5 ± 0. 5 ） was significantly lower than that of control group（ VCAM-1：82. 8 ± 0. 3, E-selectin ： 53.8 ± 0. 4） （ P 〈 0. 05）. However, there were no significant differences in women with mild pre-eclampsia （ TGF-β1 ： 70. 6 ± 0. 6, VCAM-1 ： 82. 4 ± 0. 6, E-selectin： 53.4 ± 0. 5 ） and severe ones （ TGF-β1 ： 70. 8 ±0.4,VCAM-1.82.6 ±0.5, E-selectin： 53.6 ±0.5） （P 〉0.05）; （2） The level of E-selectin in placental villous capillary endothelial cells of pre-eclampsia group （ 63.0± 0. 5 ） was significantly higher than that of control group （62. 6 ± 0. 4） （ P 〈 0. 05 ） , while there was no significant difference in women with mild pre-eclampsia（63.2 ±0.4） and severe pre-eclampsia （62. 9 ±0. 5 ） （ P 〉0. 05 ）. Conclusion TGF-β1, VCAM-1 and E-selectin are not only related to placenta shallow bed of pre-eclampsia, but also participate in pathogenic process of vascular endothelial damage of pre-eclampsia.
出处
《中华妇产科杂志》
CAS
CSCD
北大核心
2006年第8期514-517,共4页
Chinese Journal of Obstetrics and Gynecology
