摘要
In alkaline medium,a new flow injection chemiluminescent method has been developed for the determination of resveratrol,based on the inhibition of the chemiluminescence reaction between luminol and N-bromosuccinimide by resveratrol.Conditions of this chemiluminescent reaction have been discussed and optimized.The linear range of determination is 6.0 10-7-1.0 10-5mg/mL for resveratrol.The detection limit is 8.15 10-8mg/mL.And the relative standard deviation（RSD）（n=11） is 0.35% for 5.0 10-6mg/mL resveratrol.The method has been applied to determine the content of resveratrol in grape skin,grape wine and traditional Chinese medicine polygonumcuspidatum seib with satisfactory results.The possible mechanism of the chemiluminescence reaction was also studied.
In alkaline medium, a new flow injection chemilumineseent method has been developed for the determination of resveratrol, based on the inhibition of the chemiluminescence reaction between luminol and N - bromosuccinimide by resveratrol. Conditions of this chemiluminescent reaction have been discussed and optimized. The linear range of determination is 6.0 10^-7 - 1.0 10^-5mg/mL for resveratrol. The detection limit is 8. 15 10^-8mg/mL. And the relative standard deviation （RSD） （n = 11 ） is 0. 35% for 5.0 10^-6mg/mL resveratrol. The method has been applied to determine the content of resveratrol in grape skin, grape wine and traditional Chinese medicine polygonumcuspidatum seib with satisfactory results. The possible mechanism of the chemiluminescence reaction was also studied.
出处
《化学研究与应用》
CAS
CSCD
北大核心
2006年第8期1005-1008,共4页
Chemical Research and Application
基金
山东省中青年学术骨干和学科带头人培养基金资助项目
作者简介
联系人：王术皓（1964-），男，教授，研究方向为化学发光。Email：shuhaowang@sohu．com
