摘要
对首次在乳牛粪便中发现的疑似环孢子虫卵囊的形状、大小、抗酸染色和孢子化等形态学特征进行了观察,探讨了该原虫的纯化方法。结果显示,牛源环孢子虫卵囊呈球形,直径6～10μm。直接涂片时可见卵囊内含折光性较强的成团小球体,呈桑椹胚状,淡绿色。碘液染色不着色。经改良抗酸染色后,卵囊着色变化较大,呈淡红色、深红色或不着色。新排出的未孢子化卵囊培养后可观察到孢子化的卵囊,内含2个孢子囊,每个孢子囊内含有2个折光性较强的子孢子。这些特征与人或其他动物的环孢子虫一致。环孢子虫感染阳性的粪便经Sheather's蔗糖漂浮法浓集后,再经蔗糖密度梯度离心,可以得到较为纯净的卵囊。
Cyclospora-like oocysts were found in dairy cattle feces. Using wet mount slides, the oocyst walls appeared as well-defined non-refractile spheres measuring 6 to 10 μm in diameter, within each unsporulated oocyst was a central morula-like structure containing a variable number of inclusions. At a magnification of × 1 000, the inclusions were refractile, exhibiting a greenish tinge. Oocysts were stained variably using a modified acid-fast stains but colorless in saline wet mounts. Oocysts were concentrated and purified by sucrose flotation. Purified oocysts were suspended in equal volumes of 5% potassium dichromate solution to sporulate. The sporulated oocyst holds 2 sporocysts and each sporocyst contains 2 sporozoites, i. e. each oocyst contains a total of 4 sporozoites. All the above phenotypic characteristics were similar to those described previously in human being. This is the first cyclosporan to be described in dairy cattle.
出处
《中国兽医科学》
CAS
CSCD
北大核心
2006年第8期639-642,共4页
Chinese Veterinary Science
基金
国家自然科学基金(30371082)
广东省自然科学基金(32286)
关键词
环孢子虫样卵囊
乳牛
形态学
Cyclospora-like oocyst
dairy cattle
morphology
作者简介
肖淑敏（1979-），男，江西新余人，博士生。
李国清为通讯作者，E-mail：gqli@scau．edu．cn
