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猪肌生成抑制素功能区编码基因的原核表达与纯化 被引量：2

Prokaryotic Expression and Purification of Functional Domain Coding Gene of Swine Myostatin
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摘要 根据猪肌生成抑制素基因设计1对引物,PCR扩增出猪肌生成抑制素功能区编码基因片段,将该片段克隆至pGEM-T载体中。重组克隆质粒经序列分析,克隆序列与目的基因序列完全一致。重组克隆质粒经EcoRⅠ和BamHⅠ酶切,目的基因片段克隆到表达质粒径pGEX-4T-1,转化到大肠杆菌BL21(DE3)中,经IPTG诱导,融合蛋白占菌体可溶性总蛋白的18%以上。 According to the gene of swine myostatin, a pair of primers were designed to amplify the functional domain coding gene of swine myostatin. The PCR product was cloned into pGEM-T vector. The sequence analysis demonstrated that the cloning sequence was the functional domain coding gene of swine myostatin. The recombinant cloning vector was digested by BamHⅠ and EcoR Ⅰ . The gene fraction was cloned into the expression plasmid pGEX-4T-1 and the recombinant plasmid was transformed into the competent cell of BL21 （DE3）. Induced by IPTG , the recombinant proteins of swine myostatin were at a level approximately 18% of total soluble proteins.
作者 逄大欣 王卫芳 尚宇 欧阳红生
机构地区 吉林大学畜牧兽医学院 长春中医药大学医学院 长生基因药业有限公司
出处 《吉林农业大学学报》 CAS CSCD 北大核心 2006年第4期447-449,共3页 Journal of Jilin Agricultural University
基金 国家自然科学基金资助项目(30070563)
关键词 猪肌生成抑制素 克隆 融合蛋白 原核表达 swine myostatin cloning fusion protein prokaryotic expression
分类号 S858.28 [农业科学—临床兽医学]
作者简介 逢大欣（1970-），女，在读博士，研究方向：基因表达与调控。 通讯作者
  • 相关文献

参考文献10

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共引文献49

同被引文献17

  • 1杨松涛,夏咸柱,乔军,高玉伟,常爽,黄耕,郑明光.SARS病毒核蛋白基因的克隆、序列分析及其在E.coli中的高效表达[J].吉林农业大学学报,2005,27(3):331-334. 被引量：1
  • 2林忠平,白杰英,李彦舫.真菌免疫调节蛋白(FIP)结构与功能研究[J].辽宁师范大学学报（自然科学版）,2006,29(1):83-87. 被引量：11
  • 3许辅.金针菇免疫调节功能蛋白质[J].国立台湾大学生物资源及农学院.农业推广通讯,2005,(3):23-26.
  • 4Mcpherron A C,Lawler A M,Lee S J.Regulation of skeletal muscle mass in mice by a new TGF-β superfamily member[J].Nature,1997,387:83-89.
  • 5Lee S J,Mcpherron A C.Myostatin and the control of skeletal muscle mass[J].Curropin Genet Dev,1999,9:604-607.
  • 6Lee S J,McPherron A C.Regulation of myostatin activity and muscle growth[J].Proc Natl Acad Sci USA,2001,98:9306-9311.
  • 7Kambadur R,Sharma M,Smith T P L,et al.Mutation in myostatin (GDF-8) in Double-muscled Belgian Blue and Piedmontese cattle[J].PCR Methods Appl,1997,7(9):910-915.
  • 8Mcpherron A C,Lee S J.Double muscling in cattle due to mutations in the myostatin gene[J].Proc Natl Acad Sci USA,1997,94 (32):12457-12461.
  • 9Mcpherron A C,Lee S J.GDF-8 and GDF-9:Two new members of the transforming growth factor-β superfamily containing a novel pattern of cysteines[J].J Bio l Chem,1993,268:3444-3449.
  • 10Yang J,Ratovitski T,Brady J P,et al.Expression of myostatin pro domation results in musclar transgenic mice[J].Molecular Reprod And Development,2001,9999:1-11.

引证文献2

二级引证文献12

吉林农业大学学报
2006年 第4期

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