摘要
目的:对5个管花肉苁蓉材料进行鉴别,探索可溶性蛋白电泳指纹图谱与鉴定之间的关系,同时为建立中药材电泳图谱库提供材料。方法:研究了不同寄主的5个管花肉苁蓉材料的可溶性蛋白在SDS-PAGE条件下的电泳特性。结果:不同寄主的管花肉苁蓉可溶性蛋白的SDS-PAGE图谱分辨率高,重现性强,其多态性条带为55.56%。结论:各个材料的电泳图谱可以相互区别。不同寄主管花肉苁蓉可溶性蛋白的SDS-PAGE图谱可作为种间鉴定的依据。
Objective： To identify 5 specimens ofC .tubulosa （Schenk） R. Wight on different parasitifers, and explore the relationship between electrophoretic fingerprint and identification on soluble-protein, meanwhile providing data for establishing electrophoresis pattern library of Chinese crude drugs. Methods： The electrophoretic characteristics of solubleproteins from 5 specimens of C .tubulosa （Schenk） R. Wight on different parasitifers were studied with SDS-PAGE. Results： It indicated that reproducible and high resolvable electrophoresis patterns of soluble proteins of C . tubulosa （Schenk） R. Wight on different parasitifers by SDS-PAGE. Their degrees of polymorphism were 55.56%. Conclusion： Each specimen had its own unique band pattern distinguish from the others. The electrophoresis pattern of SDS-PAGE of soluble proteins can be regarded as protein fingerprints for identification of C.tubulosa （Schenk） R. Wight on different parasitifers.
出处
《中华中医药杂志》
CAS
CSCD
北大核心
2006年第7期402-404,共3页
China Journal of Traditional Chinese Medicine and Pharmacy
基金
重大基础研究前期研究专项(No.2004CCA01200)
作者简介
通讯作者：郭玉海，北京市海淀区圆明园西路2号
