摘要
目的：研究嗜酸性乳杆菌LA14菌株耗尽培养上清液（spent culture supernatant，SCS）对抗生素相关性腹泻小鼠肠道菌群的影响．方法：小鼠60只，随机分为正常组、模型组、SCS组、活菌组、SCS＋活菌组及自然恢复组（每组n=10）．采用ip氨苄青霉素2000mg／（kg·d），连续3d造成肠道菌群失调性腹泻动物模型．正常组和模型组小鼠于造模后即处死，取盲肠内容物进行肠道菌群分析.其余各组小鼠分别灌胃受试药物或生理盐水30mL／（kg·d）[含菌组中活菌数量为3×10^9CFU／（kg·d）】，3d后处死，取盲肠内容物进行肠道菌群分析．结果：小鼠ip氨苄青霉素造成肠道菌群失调，肠道内4种主导菌群的数量（1gCFU／L）明显改变，与正常组比较肠杆菌和肠球菌数量显著上升（10．13±0．10 vs 9．03±0．11，10．52±0．11 vs 9．11±0．09，P〈0．01），乳杆菌和双歧杆菌数量明显下降（10．51±0．07 vs 11．88±0．10，P〈0．01;10．38±0_31 vs 11．61±0．13，P〈0．05）．而灌胃给予SCS、活菌、SCS加活菌后，与自然恢复组比较肠道乳杆菌和双歧杆菌数量明显回升（乳杆菌：11．53±0．17 vs 9．74±0．37，P〈0．01：11．54±0．05，11．45±0．07 vs 9．74±0．37，P〈0．05：双歧杆菌：11．54±0．22，11．30±0．99 vs 9．51±0．52，P〈0．05：11．13±0．16 vs 9．51±0．52，P〈0．01），肠杆菌和肠球菌数量下降（肠杆菌：9．42±0．22，9．50±0．06，9．22±0．39 vs 9．97±0．61，P〈0．05；肠球菌：9．48±0．20，9．45±0．16，9．37±0．21 vs 9．89±0．43．P〈0．05）．结论：嗜酸性乳杆菌活菌和SCS均能调整抗生素相关性腹泻小鼠肠道菌群失调，对乳杆菌和双歧杆菌有显著扶持作用．
AIM： To study the effects of the spent culture supernatant （SCS） of Lactobacillus acidophilus strain LA14 on the intestinal flora in mice with antibiotic-associated diarrhoea.
METHODS： Sixty mice were randomly and averagely divided into 6 groups： normal control group, model group, SCS group, living bacteria group, SCS plus living bacteria group, and spontaneous recovery group. The animal model of the intestinal flora imbalance was made by intraperitoneal administration of ampicfllin 2000 mg/（kg·d） for 3 d. Then the mice in normal control group and model group were killed, while the mice in the other groups were administered （ig） with the corresponding drugs or normal saline （30 mL/kg per day, the adopted concentration of living bacteria was 3×10^9 CFU/kg per day） for another 3 d. At the end of the 3^rd day, all the mice were killed and the floras in the contents of the caecum were analyzed.
RESULTS： Intestinal flora imbalance occurred in the mice administered with ampicillin and the numbers （lgCFU/L） of four main intestinal floras changed obviously. In comparison with those in normal control group, the numbers of Escherichia coli and Enterococcus were significantly increased （10.13±0.10 vs 9.03±0.11, P〈0.01; 10.52±0.11 vs 9.11±0.09, P〈0.01）, while the numbers of Lactobacillus and Bifidobacterium were remarkably decreased （10.51±0.07 vs 11.88±0.10, P〈0.01; 10.38±0.31 vs 11.61±0.13, P〈0.05）. After the mice were administered with SCS, living bacteria and SCS plus living bacteria, the numbers （compared with those in spontaneous recovery group） of Lactobacillus and Bifidobacterium were remarkably increased （Lactobacillus： 11.53 ± 0.17 vs 9.74 ± 0.37, P〈0.01; 11.54±0.05, 11.45 ±0.07 vs 9.74±0.37, P〈0.05; Bifidobacterium： 11.54±0.22,11.30±0.99 vs 9.51±0.52, P〈0.05; 11.13±0.16 vs 9.51±0.52, P〈0.01）, while the numbers of Escherichia coli and Enterococcus were significantly decreased again （Escherichia coli： 9.42±0.22, 9.50±0.06, 9.22±0.39 vs 9.97±0.61,P〈0.05; Enterococcus： 9.48 ±0.20, 9.45±0.16, 9.37±0.21 vs 9.89±0.43,P〈0.05）.
CONCLUSION： Both living bacteria and SCS of Lactobacillus acidophilus can regulate the intestinal flora imbalance in mice with antibioticassociated diarrhoea, and help to recover the numbers of Lactobacillus and Bifidobacterium in the intestinal tract.
出处
《世界华人消化杂志》
CAS
北大核心
2006年第19期1870-1873,共4页
World Chinese Journal of Digestology
基金
大连大学科技发展基金
No.050016
关键词
嗜酸性乳杆菌
耗尽培养上清液
抗生素相关性腹泻
肠道菌群
Lactobacillus acidophilus
Spent culturesupernatant
Antibiotic-associated diarrhoea
Intestinal flora
作者简介
罗学娅．1989年贵阳医学院硕士，教授，主要从事天然药物研制与开发研究．通讯作者：罗学娅，116622,辽宁省大连市开发区,大连大学医学院药理学教研室．xxyluo@sina.com. 电话：0411-87402375.传真：0411-87402053
