摘要
目的:研究HBV家族聚集性感染者的基因型和基因变异特征．方法:选择HBV聚集性感染家族成员内部基因型完全相同的共27个家族84人,其中HBV 携带者(ASC)29例;慢性活动性肝炎(CAH)23 例;肝硬化(LC)21例;肝细胞癌(HCC)11例;用基因芯片法检验前C1896和BCP1762,1764双突变;用测序法检验HBV基因型,并设相应基因型的HBV感染对照组．结果:B型HBV家族聚集感染者ASC率显著大于C型家族(X2=5．0734,P=0．0243,P<0．05); 其BCP1762．1764双突变的发生率也显著低于 C基因型(X2=22．4000,P=0．0000,P<0．001)．两种基因型的家族聚集感染者组与相应的对照组相比在疾病临床类型方面均无显著差异．结论:检验HBV家族聚集感染者的基因型和／或BCP区1762、1764基因的双突变可以协助疾病的预测分析．
AIM： To investigate the genotypes and gene mutation of hepatitis B virus （HBV） in family infection. METHODS： Eighty-four members from 27 families, whose internal genotypes were completely the same, were selected, including 29 cases of asymptomatic HBV carriers （ASC）, 23 cases of chronic active hepatitis （CAH）, 21 cases of liver cirrhosis （LC） and 11 cases of hepatocellular carcinoma （HCC）. Gene chip technique was applied to detect the mutation in HBV pre-core region （nt1896）, and basic core promoter （BCP, nt1762, nt1764） and DNA sequencing technique was used to detect the genotypes of HBV. The control group of corresponding genotypes was also designed. RESULTS： The rate of ASC with genotype B family infection was significantly higher than that with genotype C infection （χ^2=5.0734, P = 0.0243, P 〈 0.05）, but the rate of BCP1762 and 1764 double mutation was significantly lower than that with genotype C （χ^2 = 22.4000, P = 0.0000, P 〈 0.001）. The clinical types of patients with genotype B and C family infection were not significantly different from those of the corresponding controls. CONCLUSION： It is helpful to detect the genotypes and BCP1762, 1764 double mutation of HBV in family infection in the predictive analysis of the disease.
出处
《世界华人消化杂志》
CAS
北大核心
2006年第18期1833-1835,共3页
World Chinese Journal of Digestology
作者简介
通讯作者：刘成永，221004，江苏省徐州市．传染病医院检验科．liucy0913@126．com.电活：0516-83668716传真：0516-83668717
