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胡萝卜筛选标记选择及遗传转化

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摘要 以常用的筛选剂潮霉素(Hyg)、除草剂Basta和卡那霉素(Kan)对胡萝卜愈伤组织进行敏感性试验,结果表明:胡萝卜愈伤组织对潮霉素和除草剂Basta非常敏感,筛选浓度分别以8mg/L和2mg/L较为合适;卡那霉素合适的筛选浓度应为150mg/L。以带bar基因的质粒载体对胡萝卜愈伤组织进行遗传转化,获得抗性愈伤组织和再生植株。
出处 《北方园艺》 CAS 北大核心 2006年第4期24-26,共3页 Northern Horticulture
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