摘要
目的建立测定花芪通脉颗粒中灯盏乙素含量的方法。方法采用反相高效液相色谱法RP—HPLC，ZORBAX-C18（4．6mm×150mm，5μm）色谱柱，甲醇-0.01mol·L^-1 磷酸缓冲液PH为5．2（35：65）为流动相，检测波长335nm，流速0．8ml·min^-1，枉温25℃。结果灯盏乙素在0．74—3．70μg范围内与峰面积呈良好线性关系，r=0．9996，灯盏乙素平均加样回收率为97．4％，RSD为0．98％（n=5）。结论：该方法可靠性高，准确性和重现性好，适用于花芪通脉颗粒中灯盏乙素的含量测定。
Objective To establish a simple and rapid reversed -phase high performance liquid chromatography method for the baseline separation, identification and assay of scutellarin in Huaqi Tongmai Granula. Methods The analytical column was a ZOD- BAX-CIs （4.6 mm × 150 mm,5 μm）filled with a 5 mm stationary phase. The mobile phase Consistent of methanol - （0.01 mol - L^-1 ）phosphate solution PH5.2（35：65）. The flow - rate was 0.8 ml·L^-1. The injection volume was 20 μl. The Detector wavelength was set at 335 nm, and the column temperature was 25℃. Results The calibration curve was linear over the range of 0.74 - 3.7 μg（ r = 0. 999 6）. The average recovery of the method was 97.4%, RSD was 0.98% （n = 5 ）. Conclusion The proposed method would be used in the quantitative analysis of scutellarin in Huaqi Tongmai Granula and the method is convenient,economical and accurate with good reproducibility.
出处
《时珍国医国药》
CAS
CSCD
北大核心
2006年第7期1206-1207,共2页
Lishizhen Medicine and Materia Medica Research
作者简介
周军（1973-），男（汉族），新疆乌鲁木齐人，现任中国人民解放军第四军医大学药学系化学教研室讲师，硕士学位，主要从事天然药物化学工作．
通讯作者简介：孙晓莉（1957-），女（汉族），吉林长春人，现任中国人民解放军第四军医大学药学系化学教研室教授，博士学位，主要从事天然药物化学分离与合成研究工作．
