摘要
将分离和体外扩增的小鼠胚芽干细胞,用BrdU标记后,经尾静脉同种移植于CCL4制备慢性肝损伤小鼠体内。分别于移植后2w、4w取出肝脏,进行连续冰冻切片,用免疫组织化学和免疫荧光双重染色检测移植细胞在受体小鼠肝内的存活、分布和肝细胞特异性白蛋白的表达;用PAS染色检测移植细胞的糖原代谢;HE染色观察移植动物肝组织的结构变化。探索胚芽干细胞在慢性损伤肝脏中的存活、分化和治疗作用。实验结果表明:胚芽干细胞经静脉移植后可以进入损伤肝脏中,并分化为肝样细胞和肝小叶内的其他细胞,并明显改善损伤肝脏的组织结构,具有较好的治疗作用。
The EGCs were proliferated and labeled with 5-bromo-2-deoxyuridine （BrdU）, then transplanted into the acute damaged liver by CCL4 through tail vein. 2 and 4 weeks later, the liver was extracted and 101μm-cryostat continuous sections were obtained. The existing and differentiation of the transplanted cells were identified by immunohistochemistry, immunoflorescence double staining and PAS histochemistry for BrdU and hepatic-specific albumin （ALB）, and the glycogen. The BrdU positive cells were found in the chronic damaged liver, some cells were BrdU and ALB double positive and PAS positive staining , the structures of damaged liver were also significantly improved. We demonstrated that the transplanted EGCs could be incorporated into the chronic damaged liver and differentiated into hepatocytes in this nt, and its transplantation also could compensate for chronic liver failure. These results suggest that EGCs can be used for potential cellular hepatoplasty to treat damaged liver
出处
《科技导报》
CAS
CSCD
2006年第7期54-58,共5页
Science & Technology Review
基金
山东省科技厅攻关计划项目(01BB1DADA1)
关键词
胚芽干细胞
细胞移植
慢性肝脏损伤
小鼠
embryonic germ cells
cell transplantation
damaged liver, mouse
作者简介
刘晓萍,女,青岛市青岛大学医学院,教授,主要从事干细胞的定向诱导分化及其机理的研究工作; E-mail：xpliu572001@yahoo.com.cn
高英茂（通讯作者）,男,山东大学医学院组织胚胎学教研室,教授,主要从事胚胎畸形学和干细胞研究;E-mail：he@sdu.edu.cn
