摘要
目的探讨刚地弓形虫对不同类型传代细胞的侵入能力和致凋亡作用及其差异。方法实验中采用人脐静脉内皮细胞(HUVEC)、非洲绿猴肾成纤维细胞(Vero)、小鼠单核巨噬样细胞(J774A.1)和人单核细胞系(THP-1)细胞株。采用透射电镜和吉姆萨染色法,观察刚地弓形虫RH株侵入以上细胞的能力以及细胞骨架抑制剂秋水仙素、细胞松弛素D对其影响。用流式细胞术检测弓形虫引起的细胞凋亡。结果弓形虫RH株能侵入以上4种细胞,在胞内均有纳虫泡形成。其侵入率以J774A.1和THP-1略高。细胞骨架抑制剂细胞松弛素D可以明显降低速殖子对THP-1和J774A.1细胞的侵入率,对速殖子侵入HUVEC和Vero细胞的能力影响较小。弓形虫侵入HUVEC和J774A.1细胞均可不同程度地引起细胞凋亡和坏死,但前者以凋亡为主,后者以坏死为主。结论弓形虫可能通过不同的机制侵入非吞噬性细胞和吞噬性细胞。
To explore the invasive ability of Toxoplasma gondii to the cultured cell lines and to induce apoptosis in these cells, human umbilical venous endothelial cells （HUVEC）, Vero cells, J774A. 1 cells and THP-1 cells were employed as the test cells, and the power of invasions of T. gondii into these cell lines was observed after Giemsa staining and under electron microscopy. Flow cytometry was used to investigate the apoptosis in these cells induced by T. gondii. It was demonstrated that T. gondii could invade these 4 types of cells with formation of parasitophorous vacuoles. The invasive ratios to J744A and THP-1 cells were higher than those of HUVEC and Veto cells, and the cytoskeleton inhibitor cytochalasin D could significantly reduce the invasive ratios of J744A and THP-1 cells. Different degrees of apoptosis or necrosis in HUVEC and J744A cells induced by T. gondii could be observed, mainly with apoptosis in HUVEC cells but necrosis in J744A cells. It was also evident that the difference in cell type could greatly influence the development of apoptosis or necrosis in these cell lines after induction with T. gondii. It is concluded that through different mechanisms, T. gondii can invade into various cell types, including phagocytic and non-phagocytic cells.
出处
《中国人兽共患病学报》
CAS
CSCD
北大核心
2006年第7期597-600,共4页
Chinese Journal of Zoonoses
基金
国家自然科学基金资助(30300294)
作者简介
通讯作者：严杰，Email：med_bp@zju．edu.cn
