摘要
本文报道了分布在云南的４种树蛙（峨眉泛树蛙、黑点泛树蛙、无声囊泛树蛙和棕褶树蛙）的核型、Ｃ－带及Ａｇ－ＮＯＲｓ。它们具有相同的二倍体数（２ｎ＝２６），除无声囊泛树蛙是６＋７，其余均为５＋８核型模式，种间ＳＭ的数目和顺序、次缢痕和Ａｇ－ＮＯＲＳ相互间有所差异。黑点泛树蛙Ｎｏｓ．３，１２为ＳＭ，Ａｇ－ＮＯＲｓ位于１０Ｐｉｎｔｅｒ；峨眉泛树蛙Ｎｏｓ．２，３，４，６．９为ＳＭ，随体和Ａｇ－ＮＯＲＳ在１２ｑｔｅｒ；无声囊泛树蛙无ＳＭ，Ａｇ－ＮＯＲＳ在６ｑｉｎｔｅｒ；棕褶树蛙Ｎｏｓ．２，３，４为ＳＭ，Ａｇ－ＮＯＲｓ２对，分布在１２ｑｔｅｒ和１３ｑｔｅｒ，还观察到随体联合现象。４个种的Ｃ－带正染主要在着丝点区域，次缢痕和Ａｇ－ＮＯＲＳ位置均为阴性反应。
The conventional karyotype,C-banding and Ag-NORs of four rhacophorid tree frogspecies (Those are Polypedates nigropunctatus,P.omeimontis, P. mutus and Rhacophorus feae)from Yunnan Province have been studied using the bone marrow preparing chromosome.Results showed that the same diploid number (2n= 26) and karyotypic formular they have, except for P. mutusthat have 6+7 formular, but the number and sequence of the submetacentricchromosome,secondary construction (SC) or the satellite and Ag-NORs are different eachother. In P.nigropunctatus Nos. 3,12 are SM, Ag -NORs and SC localized on 10pinter.Nos.2,3,4,6,9 are SM and the satellite, Ag-NORs are on 12qter,in P. omeimontis, without SMand on 6qinter of Ag-NORs in P. mutus, but in Rhacophorus.feae the Nos. 2,3,4 are SM, andtwo pairs Ag-NORs appeared on 12qter and 13qter.Meantimes the satellite association wasfound. All these species in which the C-positive mainly are on the centromeric regions of allchromosomes and the C-negative reaction always appeared on SC or Ag-ONRs locations.Finally the possible mechanism of karyotypic change among them have been discussed.
基金
国家自然科学基金
关键词
无尾目
树蛙科
细胞遗传学
Karyotype,C-banding,Ag-NORs,Rhacophorid frog,Yunnan
