摘要
目的:采用HPLC建立复方大青叶注射液的指纹图谱测定方法。方法:采用Diamonsil C18色谱柱,甲醇-水-磷酸为流动相进行梯度洗脱,流速1.0 mL.min-1,检测波长254 nm。结果:建立了复方大青叶注射液的HPLC对照指纹图谱,标示了注射液中的20个共有峰,并初步确定注射液的相似度阈值为0.85。结论:该方法准确、重复性好,为复方大青叶注射液的质量控制提供了依据。
Objective： To develop the chromatographic fingerprints of composite Folii Isatidis injection by HPLC. Method： The separation was performed on a Diamonsil C18 column with a mobile phase consisting of methanol-water-phosphoric acid as gradient eluent at the flow rate of 1.0 mL·min^-1. The UV detection was set at 254 nm. Result： A standard HPLC fingerprint procedure was developed for composite Folii Isatidis injection, with 20 common peaks and a similarity threshold of 9.0 established. Condusion： This method was accurate, repeatable and useful for the quality control of composite Folii Isatidis injection.
出处
《中国中药杂志》
CAS
CSCD
北大核心
2006年第13期1059-1062,共4页
China Journal of Chinese Materia Medica
基金
国家科技攻关计划项目(2001BA701A56)子项目
关键词
复方大青叶注射液
质量控制
指纹图谱
相似度
Composite Folii Isatidis injection
quality control
HPLC fingerprint
similarity
作者简介
[通讯作者]李发美，Tel：（024）23986289。E-mail：fameili@163．com
