摘要
本研究对1株鹅源H5N1亚型禽流感病毒血凝素（HA）基因进行了扩增、克隆及序列测定。结果表明：所扩增的片段长1707bp，包含完整的开放阅读框架，编码568个氨基酸。该毒株裂解位点的氨基酸序列为RRRKKR，具有高致病性的分子特征；该毒株有6个潜在糖基化位点；受体结合位点的氨基酸分别为YWIHELY，其左侧壁氨基酸为SGVSSA，右侧壁为NGQSGR。该毒株与国内外一些参考毒株的HA基因序列比较，核苷酸同源率为76.8％～98．1％，氨基酸同源率为85．7％～97．4％，属于欧亚种系。
In this study,the eDNA of HA gene of a goose avian influenza virus isolate was amplified and cloned ,and then sequenced. The sequence analysis shows that eDNA of the HA gene is composed of 1 707 bp nucleotides containing whole open reading frame of HA gene and coding 568 amino acids. Amino acids sequence RRRKKR at the cleavage site is symbol of highly pathogenic avian influenza ;There are 6 potential glycosylation sites in the influenza virus isolate;Amino acids of the receptorbinding site are YWIHELY,amino acids of left edge of the receptor-binding site are SGVSSA,amino acids of right edge of the receptor-binding site are NGQSGR. The comparative sequence analysis indicated there was 76.8M-98. 1M of nueleotide homology and 85.70%-97.4% of amino acid homology with some reference strains. This goose isolate was recognized to be originated from Euroasian lineage.
出处
《中国兽医学报》
CAS
CSCD
北大核心
2006年第4期385-386,389,共3页
Chinese Journal of Veterinary Science
基金
吉林省重大科技专项基金资助项目(20040202-3-2)
关键词
鹅
禽流感病毒
血凝素基因
序列分析
goose avian influenza virus
hemagglutinin gene
sequence analysis
作者简介
王伟利（1965-），女，高级兽医师，博士。
通讯作者
