摘要
目的:探讨马传染性贫血病毒(Equineinfectiousa-nemiavirus,EIAV)驴白细胞减毒疫苗(DLV)免疫马后,外周血单个核细胞(PBMC)中IFN-γmRNA转录水平与免疫保护应答的关系,揭示DLV的免疫保护机制。方法:应用分子克隆及实时定量RT-PCR技术,建立马PBMC中IFN-γmRNA转录水平的定量检测方法,在不同时间点定期观察4组(疫苗免疫组、阴性对照组、强毒株阳性对照组及EIAV自然感染组)12匹马PBMC中IFN-γmRNA的转录水平及分布特征,同时监测体温变化等指标。疫苗株免疫动物8个月后,用EIAV强毒株攻击,观察攻击前后IFN-γmRNA转录水平的变化。结果:疫苗免疫马在免疫期内,PBMC中IFN-γmRNA转录的量显著高于阴性对照组及自然感染组(P<0.01)。免疫后用EIAV强毒株攻击,IFN-γ转录的量继续升高,4匹免疫马均获得完全保护;强毒株阳性对照组IFN-γmRNA转录的量随疾病的进展波动,发热期明显下降。结论:本研究首次证明,EIAV减毒疫苗可诱导马PBMC中IFN-γ基因高效转录,其转录水平与DLV的免疫保护密切相关。此结果在分子水平为阐明DLV的免疫保护机制提供了新的实验依据。
AIM： To evaluate the relationship between the transcriptional level of IFN-γ mRNA in peripheral blood mononuclear cells （PBMC） and immune protective response driven by inoculated horses with donkey leukocyte attenuated vaccine of EIAV（ DLV）, and to elucidate the immune mechanism of DLV. METHODS： A real-time PCR method was established for quantitative detection of IFN-γ mRNA level from horse PBMCs. Twelve horses were divided into vaccination group, healthy control group, challenging control group and EIAV natural infection group. The transcriptional level and distribution of IFN-γ mRNA in PBMCs were analyzed. The temperature and other parameters of the inoculated horses were monitored or a daily basis. Horses inoculated with DLV vaccines were challengedpostinoculated 8 months, and the change of transcriptional level of IFN-γ mRNA during pre/post-challenge was evaluated. RESULTS： Compared with the healthy control and natural infection groug. The transcriptional level of IFN-γ mRNA was much higher in vaccination group （P〈0.01）, and kept increasing even after challenging with virulent EIAV strains.In the challenging control group, the transcriptional level of IFN--γ mRNA fluctuated with the development of disease and reduced markedly during febrific episodes. CONCLUSION： This study for the first time reveals that EIAV-attenuated vaccine could induce high level transcription of IFN-γmRNA, with is associated closely with immune protective response induced by DLV. This finding provides some clues to elucidate the mechanism of immunity protection of DLV at the molecular level.
出处
《细胞与分子免疫学杂志》
CAS
CSCD
北大核心
2006年第4期436-439,共4页
Chinese Journal of Cellular and Molecular Immunology
基金
国家自然科学基金资助项目(30371319)
科技部基础研究重大项目前期研究专项(2001CCA00600)
国家自然科学基金青年基金资助项目(30200200)
关键词
马传染性贫血病毒
驴白细胞减毒疫苗
马γ干扰素
转录
equine infectious anemia virus
donkey leucocyte-attenuated vaccine
equine IFN--γ
transcription
作者简介
王盈（1959-），女，黑龙江哈尔滨人，研究员，博士Tel：021-64370055；E-mail：wangying2000@163．com
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