摘要
以大蒜根尖分生细胞为材料研究1,2,4三氯苯(1,2,4TCB)对有丝分裂的影响.结果表明,1,2,4TCB使大蒜根尖分生细胞有丝分裂指数下降,且呈现明显的剂量效应和时间效应关系;1,2,4TCB使有丝分裂前期细胞所占比例相对增加,中期、后期、末期细胞相对减少;与后期、末期相比,中期细胞的减幅更大;1,2,4TCB各处理组的微核细胞发生率与对照组相比均没有统计学差异;双核细胞发生率在高浓度处理组与对照组相比,有极显著性的统计学差异(p<0.01),并呈现一定的剂量效应和时间效应关系.研究表明,1,2,4TCB对大蒜根尖分生细胞有丝分裂具有阻滞作用,并诱发有丝分裂异常的显著升高.
The damage effects of 1,2,4-trichlorobenzene on the mitosis in root meristematic ceils of A. sativum seedlings were studied. The results showed the mitotic index in root mefistematic ceils of A. sativum seedlings were decreased in treated time - response and dose - response manners. The prophase cell ratios among the mitotic ceils were relatively increased, while the metaphase, anaphase and telaphase ceils ratios were decreased; Compared with anaphase and telophase cells, the decreasing amplitude of metaphase ceils was bigger. Compared to the control groups, the frequencies of mieronuclei in 1,2,4-trichlorobenzene groups showed no statistic difference, whereas the frequencies of binucleated cells showed distinguished statistic difference （p 〈 0.01 ）. The increasing of frequency of binucleated cells was dependent on treated time and concentrations. Conclusion： The mitosis in root meristematic ceils of A. sativum seedlings were obviously changed after the treatment of 1,2,4-trichlorobenzene, and the abnormal division were increased.
出处
《环境科学学报》
CAS
CSCD
北大核心
2006年第6期962-967,共6页
Acta Scientiae Circumstantiae
基金
"985"工程二期项目(No.2005900043172000)
中山大学重点资助项目(No.320003253282)~~
关键词
大蒜
1
2
4-三氯苯
有丝分裂指数
微核
双核
Allium sativum
1,2,4-trlchlorobenzene
mitosis index
micronuclei
binucleated cells
作者简介
杜青平（1973-）,女,讲师（博士）,E-mail：qpdu@sxu.edu.cn
贾晓珊（1962-）,男,中山大学教授,博士生导师.目前主要研究方向为：环境微生物、环境生物治理技术和危险污染物管理与调控,E-mail：eesjxs@zsu.edu.cn,Tel：020-84114545,通信作者
