摘要
目的观察不同浓度舒芬太尼或氯胺酮预先给药对成人静脉血辅助性T细胞(Th细胞)分化的影响。方法采集22名健康志愿者外周静脉血20 ml。进行全血和外周血单个核细胞 (PBMCs)培养。每一份血样均用于下列各组研究:空白对照组(C组)、不同浓度舒芬太尼组[0．05ng／ ml(S1组)、0．5．g／ml(S2组)、5 ng／ml(S3组)]及不同浓度氯胺酮组[100 ng／ml(K1组)、500 ng／ml(K2组)、 2 500 ng/ml(K3组)]。培养的全血和PBMCs在体外均用相应浓度药物预先作用24 h,然后加入相应的刺激剂(全血:氟波醇乙酯、离子霉素及蛋白质转运抑制剂,刺激4 h;PBMCs:植物血凝素,刺激48 h)。刺激结束后,采用流式细胞技术测定全血及PBMCs中20 000个淋巴细胞中Th1及Th2亚群比例,并计算Th1细胞与Th2细胞的比值(Th1／Th2)。结果与C组比较,S2组及S3组Th1亚群比例下降,Th2亚群比例升高,S3组Th1/Th2下降;K2组及K3组Th1和Th2亚群比例均下降,K3组Th1/Th2升高(P< 0．05)。S1及K1组两种药物的上述作用均不明显。结论舒芬太尼可促使Th细胞向Th2细胞分化, 氯胺酮则对Th1和Th2细胞的分化均有抑制作用,且以抑制Th2的程度更明显,以上作用均呈剂量依赖性。
Objective To investigate the effects of pretreatment with different concentrations of sufentanil of ketamine on the differentiation of human helper T cells in vitro. Methods Twenty-two healthy volunteers （ 11 males, 11 females） aged 20-45 yrs were enrolled in this study. In each volunteer 20 ml of blood was taken from peripheral vein and divided into 7 groups： control group （0.9% NaCl）, 3 sufentanil groups （0.05, 0.5, 5.0 ng ＂ml^-1） and 3 ketamine groups （100, 500, 2 500 ng＇ml^-1） .Whole blood and mononuclear cells from peripheral blood （PBMCs） were incubated in the presence of 0.9% NaCl or different concentrations of sufentanil or ketamine for 24 h. Then the stimulants-phorbolmyristate ＋ lonomycin ＋ glgistap （ inhibitor of intracellular protein transport） were added to whole blood and phytohemagglutinin （PHA） was add to PBMCs. The whole blood was incubated for another 4h and PBMCs were incubated for another 48 h. Then the T-lymphoeytes were collected for determination of intracellular level of IFN-y（ as a marker of Thl cells） and IL-4 （ as a marker of Th2 cells） in the whole blood using three-color flow cytometry and the expression of CCR5 ＋ （ as a marker of Thl cells） and CCR3 ＋ （ as a marker of Th2 cells） in PBMCs. The Thl/Th2 ratio was calculated. Results In sufentanil 0.5 and 5.0 ng· ml^-1 groups the percentage of Th2 cells was significantly increased while the percentage of Thl cells and Thl/Th2 ratio were significantly decreased. In ketamine 500 and 2 500 ng·ml^- 1 groups the percentage of both Thl and Th2 cells were significantly decreased and the Thl/Th2 ratio was significantly increased. Conclusion Sufentanil can encourage helper cells to differentiate into Th2 cells while ketamine inhibit the helper cells to differentiate into Thl and Th2 cells, especially the Th2 cells in a dose-dependent manner.
出处
《中华麻醉学杂志》
CAS
CSCD
北大核心
2006年第5期397-400,共4页
Chinese Journal of Anesthesiology
基金
南京医科大学基金资助项目(NY03025)江苏省医学重点人才项目(RC2002058)
作者简介
通信作者：钱燕宁，210029 南京医科大学第一附属医院麻醉科（E—mail：yanning—qian@yahoo．com．cn）
