摘要
目的:模拟“高住低训”条件,观察低氧和长时间游泳运动对小鼠骨骼肌低氧诱导因子1α及乳酸脱氢酶、琥珀酸脱氢酶活性的影响。方法:实验于2004-06/08在华东师范大学生物化学实验室完成。选择雄性成年昆明小鼠18只,适应性训练1周后,按随机数字表法分为3组,即低氧对照组、低氧训练组和常氧对照组,每组6只。制备模拟低氧设备,低氧对照组进行8周的低氧适应,8h/次,5次/周,氧浓度控制在156～161mL/L。低氧训练组进行8周的低氧适应和常氧游泳训练,每次先进行8h的低氧适应后再进行1h的常氧游泳训练,5次/周。常氧对照组按常规饲养。8周后,3组小鼠均在一次性力竭运动后立即麻醉断头处死,取股外侧肌,分为两块,一块测定乳酸脱氢酶和琥珀酸脱氢酶活性;另一块测定细胞中低氧诱导因子1α蛋白含量(免疫组织化学法)。结果:饲养过程中常氧对照组脱失1只,进入结果分析低氧对照组、低氧训练组和常氧对照组分别为6,6,5只小鼠。①常氧对照组小鼠骨骼肌细胞内低氧诱导因子1α蛋白几乎无表达,而低氧训练组和低氧对照组小鼠骨骼肌细胞内低氧诱导因子1α蛋白含量较高,两组均有6个以上目标蛋白。②低氧对照组、低氧训练组小鼠骨骼肌乳酸脱氢酶和琥珀酸脱氢酶的活性均有显著高于常氧对照组[(128.49±11.83,145.37±14.33,93.65±15.32)μkat/g;(223.21±51.01,252.05±48.18,99.02±32.67)nkat/g(P<0.01)],其中低氧训练组显著高于低氧对照组(P<0.01)。结论:在模拟“高住低训”条件下,低氧加长时间大负荷运动能够促进低氧诱导因子1α蛋白的表达,提高骨骼肌糖的有氧代谢酶的活性,有利于增强机体运动功能。而且骨骼肌低氧诱导因子1α蛋白含量与乳酸脱氢酶、琥珀酸脱氢酶活性可能存在着一定的关系。
AIM： To observe the effect of hypoxia and long time swimming on hypoxia-inducible factor 1 α （HIF-1α） and lactate dehydrogenase （LDH） and succinic dehydrogenase （SDH） activity in skeletal muscle of mice under simulated ＂Living high-training low （Hi-Lo）＂ condition.
METHODS： The experiment was carried out in the biochemical laboratory of East China Normal University from June to August 2004, Eighteen male adult Kunming mice were selected and divided into three groups after 1 week adaptive training： hypoxia control （HC） group, hypoxia （HT） training group and normoxia control （NC） group with 6 mice in each group. After establishment of simulated hypexia equipment, the HC group was stimulated by hypexia for 8 weeks, 5 times per week, and 8 hours for each time with concentration of 156-161 mL/L. The lit group was trained by swimming in normal oxygen environment based on the hypoxia for 8 weeks during which the examinees were swimming in normal oxygen condition for 1 hour, five times for every week after 8 hours of hypoxia. The NC group was living in normal environment. Eight weeks later, the mice were killed under anesthesia after disposable exhausted sports to take the lateral femoral muscle and divide into two parts, one of which was used for measurement of LDH and SDH activity, the other for the assessment of HIF-1αprotein level by immunohistochemical method.
RESULTS： A total of 6, 6 and 5 mice in the HC, HT and NC groups, respectively were involved in the result analysis except for one mouse in the NC group dropped during raising. （1）There was little expression of HIF- 1α protein in the skeletal muscle of the NC group, while a great amount of HIF-1α protein were found in the HC and HT groups, in which there were more than 6 target proteins. （2）The LDH and SDH activity of the HC and HT groups were obviously higher than that of the NC group [（128.49 ±11.83, 145.37±14.33, 93.65±15.32） μkat/g; （223.21±51.01, 252.05±48.18, 99.02±32.67） nkat/g; P 〈 0.01]; and the HT group was significantly higher than the HC group （P 〈 0.01）.
CONCLUSION： The findings demonstrate that hypoxia and long-time training with heavy burden under ＂Hi-Lo＂ environment can increase the expressions of HIF-1α protein, improve glucose metabolism and enhance their motor function. Moreover, there was some correlation between HIF-1α protein level and the activity of LDH and SDH.
出处
《中国临床康复》
CSCD
北大核心
2006年第28期82-84,共3页
Chinese Journal of Clinical Rehabilitation
基金
上海市体育局立项课题(44030750)~~
作者简介
李世昌，男，1956年生，浙江省宁波市人，汉族，1982年华东师范大学毕业，教授，从事运动人体科学方面的研究。
