摘要
目的观察BNX小鼠生物学特性及肿瘤生长转移情况,为BNX小鼠的推广与应用提供基础资料。方法应用定期测定BNX小鼠体重、统计繁殖特性和检测BNX小鼠血液生理与生化指标的方法,初步探索BNX小鼠的生物学特性;应用原位移植和皮下移植的方法分别研究BNX小鼠在人胃癌(MKN-45)和人黑色素瘤(SCI-375)肿瘤动物模型研究中的应用。结果4～30周龄BNX小鼠的体重性别间差异有统计学意义(P<0.05);外周血常规指标中BNX小鼠的白细胞数只及正常小鼠的25%,而外周血清生化指标与正常小鼠相比没有明显的差异;人胃癌(MKN-45)BNX小鼠原位移植瘤模型和人黑色素瘤(SCI-375)BNX小鼠皮下移植瘤模型的转移率分别高于T细胞免疫缺陷的裸小鼠和T、B细胞联合免疫缺陷的SCID小鼠。结论BNX小鼠比一般的免疫缺陷动物更适合应用于肿瘤转移模型的研究。
Objective To supply some basic data for extension and application of BNX mice through observation on generally biological properties and peculiarity of tumor growth and metastasis after tumor implantation. Methods By the method of periodically measuring the weight, analyzing breeding character, graphing growth curve, examining hematological and blood biochemical parameters,we explored biological characteristics of BNX mice. At the same time ,we also established tumor animal models of human gastric tumor （MKN-45） and human melanoma （SCI-375） in BNX mice through orthotopic and subcutaneous tumor implantation. Results It showed that there was a statistically significant difference of body weight between male and female BNX mice within 4--30 weeks （P〈0.05）. The number of WBC in peripheral blood haematology of BNX mice was only equal to twenty-five percent of that of normal mice,while no statistically significant differences in biochemical parameters was observed between BNX mice and normal mice. The metastatic rates oforthotopic implant models of human gastric tumor （MKN-45） and subcutaneous implant models of human melanoma （SCI-375） in BNX mice were much higher than in nude mice and SCID mice.Conclusions These results suggest that BNX mice are more suitable for the study of establishment of tumor metastatic models compared with nude mice and SCID mice.
出处
《实验动物与比较医学》
CAS
2006年第2期75-79,共5页
Laboratory Animal and Comparative Medicine
基金
上海市科委医学引导项目(编号:044119738)
作者简介
范士明（1977-）男，硕士研究生，研究方向：免疫缺陷动物繁育及肿瘤模型的建立
[通讯作者]姚明（1959-）男，研究员，研究方向：肿瘤动物模型的建立及实验研究。
