摘要
目的探讨原发性高血压患者血管紧张素1型受体(AT1R)基因多态性与血管紧张素Ⅱ(AngⅡ)、内皮素(ET)的相关性。方法应用PCR-RFLP技术检测64例原发性高血压患者与正常对照组61名健康受试者AT1R基因多态性,同时测定其AngⅡ和ET,并进行对照统计学分析。结果①EH组与正常对照组间血浆AngⅡ、ET水平差异显著(分别为t=5.56,P<0.01和t=5.39,P<0.01);②EH组和正常对照组之间的AT1R基因型频率有显著的差异(χ2=10.32,P<0.01),两组间C等位基因频率亦有显著差异(χ2=8.71,P<0.01);③AngⅡ在AA、AC和CC 3种基因型之间无显著性差异,而ET在3种基因型之间具有显著性差异,ET活性在CC型>AC和AA型。结论AT1R基因1166位点的变异参与高血压的发病,C等位基因可能是易感基因;AT1R基因A1166C位点变异可能部分通过升高ET而参与高血压的发病。
Objective To investigate the relationship between polymorphism of angiotensin Ⅱ type I receptor （ATIR） gene and angiotensin Ⅱ （ Ang Ⅱ ）, endothelin （ET） in patients of essential hypertension（EH）. Methods Polymorphism of AT1R, AngⅡ and ET were studied in 64 eases of Hypertension and by PCR-RFLP and radioimmunoassay, respectively; 61 normotensive patients were served as control Results ①Serum Ang Ⅱ and ET were increased significantly in EH group compared with that in the control one （t=5.56, P 〈0. 01 vs t =5.39, P 〈0. 01, respectively） ;②The frequencies of AT1 R genotype and C allele of AT1R gene of EH patients were higher than those of control （ χ^2 = 10. 32, P 〈 0.01 vs ^2 = 8.71, P 〈 0.01, respectively） ; ③There is no difference in serum Ang Ⅱ , but markedly difference in serum ET among the patients of AA, AC and CC genotype, and the activity of ET was higher in CC genotype than that in AC and AA genotype. Conclusions The variability of A1166C in AT1R gene contributes to the occurrence of EH, and C allele may be predisposing gene; The variability of A1166C in ATIR gene may partly eontribute to the EH through the increase of ET.
出处
《遵义医学院学报》
2006年第1期25-27,共3页
Journal of Zunyi Medical University
关键词
高血压
基因多态性
血管紧张素1型受体
血管紧张素Ⅱ
内皮素
essential hypertension
gene polymorphism
angiotensin Ⅱ type 1 receptor
angiotensin Ⅱ
endothelin
作者简介
赵然尊（1980-）,男,山东省人,研究方向：高血压病的发病机制与冠心病介入治疗.
