摘要
分离豚鼠外周血淋巴细胞加入ＣｏｎＡ在体外培养后，收集上清液。１０－５ｍｏｌ／Ｌ的组胺不能诱导正常密度嗜酸细胞（ＮＥｏ）转变为低密度嗜酸细胞（ＨＥｏ），但Ｅｏｓ经淋巴细胞培养上清预处理后，组胺能明显地刺激ＮＥｏ转变为ＨＥｏ（Ｐ＜０．０５），１０－６ｍｏｌ／ＬＰＡＦ能诱导１５．９％的ＮＥｏ转变为ＨＥｏ，而经淋巴细胞培养上清预处理能显著增强ＰＡＦ的作用。淋巴细胞培养上清与Ｅｏｓ悬液１：１比例温育３０ｍｉｎ，对Ｅｏｓ密度无显著影响，但温育４８ｈ能显著地诱导ＮＥｏ转变为ＨＥｏ（Ｐ＜０．０１）。同样，淋巴细胞培养上清与Ｅｏｓ作用３０ｍｉｎ，不能诱导Ｅｏｓ脱颗粒，作用４８ｈ能诱导Ｅｏｓ释放约２８％的Ｅｏｓ过氧化物酶（ＥＰｏ）。以上表明淋巴细胞产物能致敏Ｅｏｓ，增强炎性介质刺激ＨＥｏ产生的能力。淋巴细胞产物诱导ＨＥｏ产生的作用较ＰＡＦ等缓慢，这种作用可能是刺激Ｅｏｓ脱颗粒的结果。
Perepheral blood lymphocytes were separated from guinea pigs and cultured with 0.5% ConA in vitro.The culture supernatant was collected 24 h later.Histamine on dosage of 10-6mol/L couldn't induce the changes of phenotype on density of eosinophils from guinea pigs treated by polymaxin'B.However,after eosinophils were pretreated by culture with lymphocytes culture supernatant,histamine converted slightly but stactistically normodense eosinophils to hypodense eosinophils (P<0.05).Platelet-activating factor(PAF)also changed phenotype of density of eosinophils (P<0.05).Moreover,lymphocytes culture supernatant primed the effect of PAF on development of hypodense eosinophils(P<0.05).lymphocytes culture supernatant could not stimulated eosinphils to degranulate by treatment for 30 min. But,lymphocytes culture supernatant induced eosinophils to release average 28% eosinophils peroxidase,a granular protein after treatment for 48 h.We conclude that the product of lymphocytes could prime and'induce normodense eosinophils convert to hypodense eosinophils.
出处
《上海免疫学杂志》
CSCD
北大核心
1996年第2期100-103,共4页
Shanghai Journal of Immunology
基金
国家自然科学基金
