摘要
目的观察红景天对兔股动脉粥样硬化模型斑块稳定性的影响,并初步探索其机制。方法将新西兰兔以高脂饮食和股动脉球囊扩张法建立动脉粥样硬化模型,随机分为空白对照组、辛伐他汀组、红景天组。测定用药前后血脂、血清MMP-3水平;观察药物干预后组织病理,进行免疫组化检测。结果红景天组与辛伐他汀组的血清胆固醇和血清LDL-C均明显低于空白对照组,血清MMP-3水平也明显下降(P<0.05或P<0.01);红景天组与辛伐他汀组均能明显减少斑块内CD34、VEGF、MMP-3和胶原的阳性染色面积,与空白对照组相比有明显差异(P<0.05或P<0.01);红景天组的斑块内胶原面积显著大于辛伐他汀组(P<0.01),而斑块内CD34阳性面积则显著低于辛伐他汀组(P<0.01)。此外,红景天组能明显减少α平滑肌肌动蛋白抗体阳性染色区域(P<0.01),但辛伐他汀组对α平滑肌肌动蛋白抗体阳性染色区域影响不大。结论红景天能有效抑制斑块内新生血管生长,降低血清LDL-C和总胆固醇,并且能减少斑块内细胞外基质的降解,有利于稳定动脉粥样硬化斑块。
Objective To investigate the effect of Rhodiola rosea and Simvastatin on the arteriosclerosis rabbit model.Methods Arteiosclerosis rabbit model was established with high-fat diet and angioplasty, then 30 New Zealand rabbits were divided into control group, Rhodiola rosea group and Simvastatin group. The blood lipid, serum MMP-3 and pathological histology were observed. Results Rhodiola rosea and Simvastatin could both decrease serum total cholesterol and LDL-C, serum MMP-3 also dropped（P〈0.05 or P〈0.01）.Two medicines could both decrease CD34, VEGF, MMP-3 and collagen（P〈 0.05 or P〈 0.01）positive area in the arteriosclerotic plaque by means of immunohistochemistry techniques. The collagen positive area was decreased less and CD34 positive area dropped more in Rhodiola rusea group than that in Simvastatin group（P〈 0.01 ）. The αSMA positive area was little affected by Simvastatin but significantly decreased by Rhodiola rosea（P〈 0.01）.Conclusion Rhodiola rosea could inhibit angiogenesis and degradation of partial extracellular matrix in the arteriosclerosis rabbit model. These mechanisms might stabilize arteriosclerotic plaque .
出处
《上海中医药杂志》
北大核心
2006年第6期66-68,共3页
Shanghai Journal of Traditional Chinese Medicine
基金
上海市科委基础研究基金资助项目(03JC14018)
作者简介
李天奇（1978-），男，上海人，在读博士生，医师，主要从事冠心病的基础研究。
