摘要
目的:探讨不同大肠癌细胞株的形貌结构差异与其转移特性的相关性．方法:对3种遗传背景一致,具有不同转移特性的大肠癌细胞株SW620(淋巴道转移)、 SW480(血道和淋巴道转移)SW480／M(肝转移)细胞的形态结构进行原子力显微镜和光镜观察,以及应用免疫组化技术检测CEA、 CD44、Fascin、PCNA在3种细胞的表达．结果:3个细胞系主要由4种形态细胞构成,包括类圆形细胞、单极细胞、纤维母细胞样细胞及瘤巨细胞．SW620瘤细胞纤维母细胞样细胞较多,表面有较多的微细绒毛和细长胞突,瘤细胞间黏附不紧密．SW480／M细胞具有上皮细胞分化的特点,主要由类圆形和单极细胞构成。细胞与细胞连接紧密,胞质融合,细胞膜表面特化结构不明显．SW480细胞形态介于 SW620和SW480／M之间．CEA在3种细胞均低表达:CD44和PCNA在SW480／M的表达强度明显高于SW620和SW480,差异显著(CD44v5: 26．023±2．394vs10．554±1．955,14．411± 4．826,P<0．001:PCNA:93％vs37％,40％, P<0．001)．Fascin表达在SW620与SW480／MS之间差异明显(26．507±2．471vs19．157±3．563, P<0．05),而在SW620和SW480之间无差异．结论:3种大肠癌细胞固有的表面形貌和免疫表型存在差异,可能与肿瘤细胞转移特性有关．
AIM： To investigate the correlation between the different property of metastasis and the variance of morphological architecture in human colorectal carcinoma （HCC） cell lines.
METHODS： The morphological architectures of three HCC cell lines with the identical genetic background and different property of metastasis, including SW620 （lymphatic metastasis）, SW480 （hematogenous and lymphangitic metastasis） and SW480/M （hepatic metastasis）, were observed by light microscopy and atomic force microscopy （AFM）, and the expression of carcinoembryonic antigen （CEA）, CD44v5, Fascin and proliferating cell nuclear antigen （PCNA） in those cell lines were detected by immunohistochemical method.
RESULTS： There were four types of cells, shaping as round-like, monopole, fibroblast-like, and giant, in all the three cell lines. In SW620 cell line, there were more fibroblast-like cells with many microvillus and pseudopodia on the cell surface and loose inter-cells conjunction. SW480/M consisted of round-like and monopole cells principally, which had the characteristics of epithelial differentiation. The cytoplasm margin was unclearly like plasmogamy and the specialized structure on cell membrane was unobvious. The morphology of SW480 cell was showed between SW620 and SW480/M. The level of CEA expression in all the three cell lines was lower. The expression of CD44v5 and PCNA in SW480/M were significantly higher than those in SW620 and SW480 （CD44v5：26.023 ± 2.394 vs 10.554 ± 1.955, 14.411 ±4.826, P 〈 0.001; PCNA： 93% vs 40%, 37%, P 〈 0.001）. The expression of fascin in SW620 was significantly higher than that in SW480/M （26.507 ± 2.471 vs 19.157 ± 3.563, P 〈 0.05）. However, fascin expression was not markedly different between SW620 and SW480.
CONCLUSION： Morphological architecture and immunophenotypes are different in the three types of cultivated cells, which may be related to their property of metastasis.
出处
《世界华人消化杂志》
CAS
北大核心
2006年第11期1097-1102,共6页
World Chinese Journal of Digestology
基金
国家自然科学基金资助项目
No.30370649
关键词
原子力显微镜
结直肠癌
细胞系
形态结构
转移
Atomic force microscope
Colorectalcarcinoma
Cell lines
Morphological architecture
Metastasis
作者简介
通讯作者：丁彦青，510515．广东省广州市．南方医科大学病理学教研室．电话：020-61642148传真：020-61642148
