祛瘀生新法对脑梗死大鼠治疗性血管新生作用及机制研究被引量：19

The Effect and the Mechanism of Eliminating Blood Stasis to Promote Regeneration of Blood Therapy on Therapeutic Angiogenesis in Rats with MCAO

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摘要目的从促血管新生角度研究祛瘀生新中药对脑梗死大鼠缺血半暗带的作用及其作用机理。方法将大鼠随机分为空白组、假手术组、模型组及祛瘀生新汤低剂量组、中剂量组和高剂量组,以线栓法复制脑梗死大鼠模型;各治疗组给予祛瘀生新汤灌胃,其余各组给予等量生理盐水灌胃。给药7d后进行检查。结果祛瘀生新汤各组VEGFmRNA表达水平、VEGF蛋白细胞的表达以及微血管密度均有所增强,特别是祛瘀生新汤中剂量组和大剂量组增强尤其明显。结论祛瘀生新中药可以增强线栓法脑梗死大鼠脑组织CD31阳性微血管密度,有一定的促血管新生作用,其作用可能是通过上调促血管生成因子的基因和蛋白表达来实现的。 Objective:To observe the influence of Eliminating Blood Stasis to Promote Regeneration of Blood Decoction to vascular endothelial growth factor(VEGF) of penumbra of the model rats' brain,to study the efficiency and the mechanism of Eliminating Blood Stasis and Promoting Tissue Regeneration therapy. Methods:The rats were divided into 6 groups randomly:the normal group,the sham operation group;the model group;the low dose group;the middle dose group and the high dose group.The model was made by the method of middle cerebral artery occlusion.On post operation days seven,the brain tissue samples were taken.Results:Every dose of Eliminating Blood Stasis to Promote Regeneration of Blood Decoction could increase the expression of VEGF mRNA,the expression of VEGF and the MVD,especial the middle and the high dose groups. Conclusion:Eliminating Blood Stasis to Promote Regeneration of Blood therapy could increase the MVD of CD31 positive in model rats brain through increasing the expression of angiogenic factor-VEGF,both gene and albumen level.

作者金远林王海燕邱幸凡张六通高铁祥

机构地区广东省深圳市宝安区中医院湖北中医学院

出处《中国中医急症》 2006年第6期632-633,638,共3页 Journal of Emergency in Traditional Chinese Medicine

关键词治疗性血管新生血管内皮生长因子微血管密度祛瘀生新法 Therapeutic angiogenesis vascular endothelial growth factor microvascular density Eliminating Blood Stasis to Promote Regeneration of Blood

分类号 R285.5 [医药卫生—中药学]

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