摘要
目的:探讨感觉神经肽在变应性鼻炎发病机制和疾病发展中的作用。方法:①将12只致敏兔随机分成2组:实验组8只进行致敏,对照组4只作为对照。实验组致敏后1、4周各处死4只,同时对照组各处死2只,取出鼻黏膜,制片后行免疫组织化学检查,测定P物质(SP)、降钙素基因相关肽(CGRP)的阳性率。②将24只致敏兔分成2组:蝶腭神经切断组和交感神经切除组。2组兔分别在术后1、2、4周各处死4只,取出鼻黏膜,制片后行免疫组织化学检查。结果:①实验组鼻黏膜中SP和CGRP的阳性率明显高于对照组(均P<0.01)。②蝶腭神经切断组术后1周与2周鼻黏膜中SP和CGRP阳性率差异均有统计学意义(均P<0.01),4周后二者阳性率与第1周无明显差异。交感神经切除组鼻黏膜中SP和CGRP阳性率术后1、2、4周无明显差异。结论:SP和CGRP与变应性鼻炎发病和发展有相关性。
Objective： To explore the roles of the sense neuropeptides in allergic rhinitis by observing their changes in the nasal mucosa after cutting the nasal autonomic nervous. Method：①Twelve rabbits were divided into two groups：group A（sensitized） and group B（control）. Four rabbits were killed respectively in one and four weeks in group A after being sensitized . Two rabbits were killed respectively at the same time in group B. Their nasal mucosa were collected for detecting substance P （SP） and calcitonin gene-related peptide （CGRP） by immunohistochemistry. ②Twenty-four rabbits were divided into two groups： group A（ sphenoidpalatine nerve was cut）, group B（sympathetic nerve was cut） . Four rabbits were killed respectively in one,two,and four weeks in group A and B. Their nasal mucosa were collected for immunohistochemistry examination. Result： ①SP and CGRP were apparently higher in allergic rabbits than non-allergic ones. ②SP and CGRP decreased apparently in one and two weeks in group A and appeared no difference in four weeks. There were no apparently difference in group B among four weeks. Conclusion：SP and CGRP are correlative with the occurring and developing of allergic rhinitis.
出处
《临床耳鼻咽喉科杂志》
CSCD
北大核心
2006年第11期508-510,共3页
Journal of Clinical Otorhinolaryngology
关键词
鼻炎
变应性
神经肽
P物质
降钙素基因相关肽
Rhinitis,allergic
Neuropeptide
Substance P
Calcitonin gene-related peptide
作者简介
通讯作者：石崧（Email：shisongwj@sina．com）
