摘要
经６％─１２％ＤｅｘｔｒａｎＴ７０密度梯度离心，获得了纯度较高的７ｄ龄花生幼苗下胚轴质膜和液泡膜制剂。１５０ｍｍｏｌ／ＬＮａＣｌ或１０℃低温处理花生幼苗２４ｈ，其下胚轴质膜上的Ｍｇ２＋激活的ＡＴＰａｓｅ活性分别提高了３７．６％和１７．２％；Ｃａ２＋─ＡＴＰａｓｅ活性分别提高４５．８％和３３．６％。上述盐或低温处理也提高了液泡膜上Ｍｇ２＋激活的ＡＴＰａｓｅ活性，分别为对照的１４１．２％和１１６．７％；Ｃａ２＋－ＡＴＰａｓｅ的活性也随之有所增加。盐或低温胁迫分别使质膜ＮＡＤＨ的氧化速率下降为对照的４５．９％和６６．７％；同时，质膜中ＰＩＰ２含量明显下降，仅为对照的４２％和６５％。推测，肌醇磷脂信息传递系统很可能参与了细胞对盐或低温胁迫的感受，影响ＡＴＰａｓｅ的活性及某些生理反应。
Plasma membrane and tonoplast membrane were obtained from 7-day old peanut seedling hypocotyl by 6% - 12% Dextran T70 density gradient centrifugation. When the seedlings were treated with 150 mmol/L NaCl or 10℃ for 24 hours, Mg2+-activated ATPase activity of hypocotyl plasmalemma increased 37. 6% and 17. 2%; while Ca2+-ATPase increased 45. 8% and 33. 6%. We could also measure both Mg2+-activatedATPase activity and Ca2+-ATPase activity in tonoplast membrane, and found that the above treatments stimulated Ca2+-ATPase a little and stimulated Mg2+-activated ATPase activity which were 141. 2% and 116. 7% of controls respectively. However, salt and low temperature treatments decreased NADH oxidation rates to 45. 9% and 66. 7% respectively of those of controls. At the same time, PIP2 levels of plasmalemma dropped extraordinarily, only were 42. 0% and 65. 0% of the controls. We suggested that phosphatidylinositol signaling transduction system probably took great part in cell'perception to salt and low temperature stress signals, thus influenced ATPase activities and some other physiological reactions.
出处
《西北植物学报》
CAS
CSCD
北大核心
1996年第1期17-22,共6页
Acta Botanica Boreali-Occidentalia Sinica
基金
国家自然科学基金
关键词
花生
幼苗
低温胁迫
PIP2
盐胁迫
腺苷三磷酸酶
Salt and low temperature stress, Peanut seedlings, ATPase activity, PIP2 content
