摘要
目的:研究依达拉奉对大鼠小肠缺血再灌注肠黏膜损伤的保护作用,为扩展其临床新用途提供实验依据。方法:通过建立大鼠小肠缺血再灌注模型,检测缺血再灌注1h后血清和小肠组织的超氧化物歧化酶(SOD)和丙二醛(MDA)的含量,并观察小肠黏膜病理变化。结果:小肠缺血再灌注后,血清及小肠组织中反映氧化损伤程度的MDA明显升高,抗氧化酶SOD则明显减少,小肠黏膜损伤,应用依达拉奉后能显著改善上述改变。结论:依达拉奉对肠缺血再灌注引起的肠黏膜损伤具有明显保护作用。
Objective To study the protective effect of edaravone on intestinal isehemia-reperfusion injury in rats, and offer an experimental basis for expanding its new clinical usage. Methods In male Wistar rats with or without edaravone treatment, intestinal damage was induced by damping the superior mesenteric artery for 30 minutes prior to reperfusion. Edaravone was administered via intravenous infusion 5 minutes before reperfusion was achieved by removal of the clamp. The rats were sacrificed after 60 minutes of reperfusion. SOD and MDA levels were measured as an index of mueosal injury and histological examination of hematoxylin and eosin-stained seetions was performed. Results The MDA levels in intestinal tissue and serum were significantly increased 60 minutes after reperfusion （ P 〈 0. 01）. The SOD levels, in contrast, were decreased 60 minutes after reperfusian （ P 〈 0. 01 ）. The results were eorroborated by histological evaluation of the intestinal tissue. Administration of edaravone diminished the changes of SOD and MDA levels therapeutically. Edaravone could also improve the damage of mucosa significantly. Conclusion Edaravone has been demonstrated to have prophylactic-therapeutic effect on intestinal mueosal injury induced by isehemia-reperfusion in rats by scavenging free radicals in vivo.
出处
《实用医学杂志》
CAS
2006年第10期1117-1119,共3页
The Journal of Practical Medicine
关键词
再灌注损伤
肠粘膜
活性氧
依达拉奉
Reperfusion injury Intestinal mucosa Reactive oxygen species Edaravone
