摘要
本研究测定了四川4个引进山羊品种24个个体的线粒体控制区全序列,并从GenBank获得山羊属2个野山羊种的2每控制区序列。利用MEGA2．0软件构建分子系统发育无根树。序列分析表明:山羊控制区线粒体控制全序列长度为1 212bp或1 213 bp,A+T含量占59．9％,其中64个核苷酸位点存在变异(约占5．28％),核苷酸多样度为 1．731％,这些差异共定义了16种单倍型,单倍型多样性为0．913±0．048。安哥拉山羊、波尔山羊都有自己独特的单倍型,与其他品种间都没有共享类型。使用NJ法构建了系统发育树,结果表明:2个野山羊种中,角(?)羊与家养山羊的关系相对较近,4个家养山羊品种有2个母系来源,在4个家养山羊品种内部,安哥拉山羊的分化要比其他3个品种早。
Complete sequence of mitochondrial DNA（mtDNA） control region（CR, D-loop） was sequenced in 24 individuals from 4 introduced goat （Capra hircus） breeds in China. In addition the control region sequences of 2 species of capra genus were obtained from the GenBank. Phylogenetic unrooted trees were constructed with neighbour-joining method. The complete sequence of mtDNA CR was 1 212 or 1213 bp（base pair） and A＋T content was 59.9%. The sequence alignment revealed variation in 64 nucleotide sites （about 5.28%）. High nucleotide diversity （ 1.713% ）and haplotype diversity（0.913） were manifested. All sequence polymorphic sites defined 16 haplotypes. The unique haplotypes existed within both Angora and Boer goat. Three phylogenetic tree constructed by Neighbor-joining methods had higher confidence probabilities. There was closer genetic relationship between Capra aegagrus and Capra hircus. The phylogeny showed two maternal origins in four introduced goats. Within four breeds, angora goats had significant genetic divergence than others.
出处
《中国畜牧杂志》
CAS
北大核心
2006年第9期1-4,共4页
Chinese Journal of Animal Science
基金
国家863基金项目(2002AA242051)
关键词
畜牧
兽医科学基础学科
序列变异性
MTDNA控制区
系统发育
山羊
basal disciplines of animal and veterinary science
sequence variability
mitochondrial control region
phylogeny
goat
作者简介
张红平（1971-），男，四川平昌人，副教授，博士
通讯作者
