摘要
目的 研究苏云金杆菌以色列变种(Bti)乳剂现场杀灭嗜人按蚊和中华按蚊幼虫的效果。方法 采用生物测定方法,分别确定Bti乳剂对嗜人按蚊和中华按蚊幼虫的LC50和LC95。通过现场试验,观察不同剂量组Bti乳剂对嗜人按蚊和中华按蚊幼虫的即效与持效。结果 Bti乳剂对嗜人按蚊(广西分离株)和中华按蚊(江苏分离株)Ⅲ龄幼虫的24hLC50分别为1.14μmol/L和0.15μmol/L,LC95依次为3.91μmol/L和0.46μmol/L。现场试验:对于嗜人按蚊,施药后第1、2、3d,1.5ml/m^2剂量组的平均幼虫死亡率依次为98.0%、96.0%、18.4%;3.0ml/m^2剂量组的平均幼虫死亡率分别为1000A、97.2%、90.8%。对于中华按蚊,施药后第1、2、3d,1.0ml/m^2剂量组的平均幼虫死亡率分别为98.4%、89.60A、67.6%;2.0ml/m^2剂量组平均幼虫死亡率依次为100%、99.2%、86.0%。结论Bti乳剂施药剂量1.0~3.0ml/m^2,能有效控制单季稻田按蚊幼虫孳生,持效为2~3d。
Objective To study the toxicity and efficacy of Bacillus thuringiensis var. israelensis (Bti) emulsion against Anopheles anthropophagus and Anopheles sinensis in the field. Methods Bioassays were carried out against third instar larvae of An. anthropophagus and An. sinensis to determine the median lethal concentration (LC50) and 95% lethal concentration (LC95) of Bti. Field trials were conducted in rice paddies of Hubei Province to test the effectiveness of different concentrations of the Bti emulsion. Results Bioassays against An. anthropophagus (Guangxi isolate) showed that LC50 and LC95 values were 1.14 μmol/L and 3.91μmol/L, respectively. Bioassays against An. sinensis (Jiangsu isolate) indicated that LC50 and LC95 values were 0.15μmol/L and 0.46μmol/L, respectively. Field trials against An. anthropophagus showed that, the 1.5 ml/m^2 concentration resulted in 98.0 %, 96.0 %, 18.40% of mean larval mortality on day 1, 2 and 3; and the 3.0 ml/m^2 concentration caused 100%, 97.2%, 90.8% of mean larval mortality on 3 days post-treatment. Against An. sinensis, the 1.0 ml/m^2 concentration caused 98.4%, 89.6%, 67.6% of mean larval mortality within 3 days of treatment; and the 2.0 ml/m2 dosage was still 86.00^ effective on day 3 (100%, 99.2%, 86.0%). Conclusion Dosages of 1.0-3.0 ml/m^2 are sufficient to control An. anthropophagus and An. sinensis in the field. The residual duration of Bti emulsion was 2-3 days.
出处
《中国病原生物学杂志》
CSCD
2006年第2期117-119,共3页
Journal of Pathogen Biology
基金
国家科技攻关疟疾课题(No.2004BA718B13)。
关键词
苏云金杆菌以色列变种
毒效评价
嗜人按蚊
中华按蚊
Bacillus thuringiensis var. israelensis
evaluation of toxicity and efficacy
Anopheles anthropophagus
Anopheles slnensis
作者简介
王蓉蓉(1977-),女(汉族),湖南人,2005年毕业于中国疾病预防控制中心流行病与卫生统计学专业,硕士。现为中国疾病预防控制中心寄生虫病预防控制所研究实习员,主要从事寄生虫病防治与科研工作。E-mail:wangrongrongl@sohu.com