摘要
目的:观察哮喘病人IL-8和IL-10水平的变化,探讨判断哮喘气道炎症及治疗效果的指标。方法: 选择41例哮喘病人和29名健康对照者,通过竞争性PCR检测其外周血单个核细胞IL-8 mRNA和IL-10 mRNA 的表达量。其中17例哮喘病人在抗炎治疗后也进行了上述检测。结果:哮喘组IL-8 mRNA表达量[(23．0± 5．9)pg/μg总RNA]明显高于健康对照组[(1．9±0．6)pg/μg总RNA,P<0．05]。健康对照组均有IL-10 mR- NA表达[(5．5±1．2)pg／μg总RNA];哮喘组在治疗前多数无IL-10 mRNA表达。17例哮喘病人治疗后IL-8 mRNA表达量[(7．0±1．6)pg/μg总RNA]比治疗前[(39．9±12．6)pg/μg总RNA]明显降低(P<0．05),激发试验阴性者均有IL-10 mRNA表达[(0．8±0．3)pg／μg总RNA],激发试验阳性者仅3例有IL-10 mRNA表达 [(0．2±0．6)pg／μg总RNA]。结论:动态观察IL-8水平变化,对哮喘病情判断有一定价值;而IL-10表达水平在某种程度上反映了对抗炎治疗的反应。
Objective： To determine on certain markers for evaluating the severity of airway inflammation and efficacy of anti-inflammation therapy in patients with asthma. Methods： 41 asthmatics and 29 normal subjects were enrolled and peripheral blood mononuclear cells （PBMCs） were collected. IL-8 and IL-10 mRNA levels were determined by competitive PCR. PBMCs were collected in 17 out of 41 patients after six months of inhaled Budesonide therapy. Results： the expression of IL-8 mRNA in PBMCs in asthma group [ （23.0±5.9） pg/μg total RNA] was significantly higher than that in control group [ （ 1.9 ± 0.6 ） pg/μg total RNA, P 〈 0.05 ） ]. Before treatment, IL-10 mRNA expression was found in the control group [ （ 5. 5 ± 1. 2 ） pg/μg total RNA ] but not in the majority of asthmatics. After anti-inflammation therapy, the expression level of IL-8 mRNA in PBMCs in 17 patients [ （7.0 ± 1.6） pg/μg total RNA] fell, but was still significantly higher than normal （P 〈 0.05 ）, and IL-10 mRNA became measurable in patients [ （0.8 ±0.3） pg/μg total RNA] with negative bronchial provocation tests. In contrast, only three [ （0.2±0.6） pg/μg total RNA] out of 11 patients with positive bronchial provocation tests demonstrated IL-10 mRNA expression in PBMCs. Conclusion： Quantification of IL-8 mRNA expression in PBMCs is useful in the evaluating the severity of asthma, and expression of IL-10 mRNA in PBMCs in asthmatics may be useful in evaluating the response to anti-inflammation therapy.
出处
《广州医学院学报》
2005年第6期4-7,共4页
Academic Journal of Guangzhou Medical College
基金
"九五"国家科技攻关项目(96-906-02-18)
作者简介
何梦璋（1970.12-）,男,主治医师,医学硕士.研究方向：支气管哮喘发病机制.
