摘要
采用平板稀释法从盐碱环境植物根围土样中分离得到263株细菌,从中筛选出15株在盐胁迫下对棉种的发芽率和胚根生长有促进作用的菌株,而且对不同浓度的氯化钠有一定的耐受性。其中Rs15-4菌株效果显著,发芽率提高了11.8%,平均根长和茎长分别提高了27.33%和17.77%,平均单株鲜重和干重提高了14.92%和7.2%。同时对Rs15-4菌株的作用机理进行了初步研究,结果发现:通过菌株处理后棉花子叶细胞膜透性比对照减小26.15%,在一定程度上促进了根系对N、P、K元素的吸收。
263 strains of plant rhizobacteria were isolated with the plate dilution in selected media containing the given concentration of NaCl, and 15 strains of that which could increase the germinating rate, promote growth of cotton, and tolerate stress of the different concentrations of NaCI. The effect of Rs15-4 strain on the germinating rate and promoting growth of cotton were investigated. The results showed that the germinating rate of cotton with treatment with Rs15-4 strain enhanced 11.8% .The average root and stem length of cotton increased 27.33% and 17.7%, respectively.The average fresh weight and dry weight of cotton increased 14.92% and 7.2% respectively. At the same time, the action mechanism of Rs15-4 strain to cotton was studied. It indicated that the cell membrane permeability of cotton treatment by Rs15-4 strain was of 26.15% lower than that of cotton without treatment by Rs15-4 strain. The absorption of N,P and K in cotton cell was promoted after treatment with Rs15-4 and the absorption of Na was significantly decreased.
出处
《石河子大学学报（自然科学版）》
CAS
2006年第1期79-82,共4页
Journal of Shihezi University(Natural Science)
基金
新疆特殊环境微生物重点实验室开放课题资助(XJYS0203-2005-03)
关键词
盐胁迫
解盐促生菌
筛选
棉花
salt stress
relieving-salt-stress
growth promoting
bacteria
isolation
cotton
作者简介
莫文萍（1980-）,女,硕士生,从事土壤微生态与植物健康研究.
通讯作者：李晖,女,博士, 副教授,从事分子植物病理学研究.电话：0993-2026942,e-mail：lihui-bce@shzu.edu.cn.
